Detection of Human Herpesvirus-6 in Cerebrospinal Fluid of Patients with Meningococcal Meningitis— Report of Two Cases from Gaziantep, Turkey

Tekin Karslıgil; Yasemin Zer; Mehmet Erinmez

doi:10.5152/eurjther.2021.20044

Authors

Tekin Karslıgil Department of Medical Microbiology, Gaziantep University Faculty of Medicine, Gaziantep, Turkey https://orcid.org/0000-0001-7672-3625
Yasemin Zer Department of Medical Microbiology, Gaziantep University Faculty of Medicine, Gaziantep, Turkey https://orcid.org/0000-0002-9078-9900
Mehmet Erinmez Department of Medical Microbiology, Gaziantep University Faculty of Medicine, Gaziantep, Turkey https://orcid.org/0000-0002-3570-3510

DOI:

https://doi.org/10.5152/eurjther.2021.20044

Keywords:

Meningitis, cerebrospinal fluid, meningococcemia

Abstract

Meningitis is a very severe and life-threatening clinical table. For rapid diagnosis of meningitis, DNAs or RNAs of the possible pathogens are investigated using syndromic panel-based testing, and it is very commonly used for early guidance to therapy in recent years. Depending on the specificity and sensitivity of the tests, it is possible to detect more than one pathogen. In this research, cerebrospinal fluid of two patients with early diagnosis of meningitis based on their clinical findings were tested using BioFire FilmArray Multiplex PCR (Biomerieux, France). Neisseria meningitidis and human herpesvirus-6 were codetected. For further evaluation of clinical meaning of this codetections, this case report is presented.

Metrics

Metrics Loading ...

References

Popowitch EB, O’Neill SS, Miller MB. Comparison of the Biofire FilmArray RP, Genmark eSensor RVP, Luminex xTAG RVPv1, and Luminex xTAG RVP fast multiplex assays for detection of respiratory viruses. J Clin Microbiol. 2013;51(5):1528-1533.

Pfefferle S, Christner M, Aepfelbacher M, Lu¨tgehetmann M, Rohde H. Implementation of the FilmArray ME panel in laboratory

routine using a simple sample selection strategy for diagnosis of meningitis and encephalitis. BMC Infect Dis. 2020;20(1):170.

Leber AL, Everhart K, Balada-Llasat JM, et al. Multicenter evaluation of BioFire FilmArray meningitis/encephalitis panel for detection of bacteria, viruses, and yeast in cerebrospinal fluid specimens. J Clin Microbiol. 2016;54(9):2251-2261.

Green DA, Pereira M, Miko B, Radmard S, Whittier S, Thakur K. Clinical significance of human herpesvirus 6 positivity on the FilmArray

meningitis/encephalitis panel. Clin Infect Dis. 2018;67(7):1125-1128.

Slenker AK, Royer TL, Villalobos T. Human herpesvirus 6 positivity on the FilmArray meningitis/encephalitis panel needs clinical interpretation. Clin Infect Dis. 2019;69(1):192-194.

Johri S, Gorthi SP, Anand AC. Meningococcal meningitis. Med J Armed Forces India. 2005;61(4):369-374.

Yao K, Honarmand S, Espinosa A, Akhyani N, Glaser C, Jacobson S. Detection of human herpesvirus-6 in cerebrospinal fluid of patients

with encephalitis. Ann Neurol. 2009;65(3):257-267.

Du B, Hua C, Xia Y, et al. Evaluation of BioFire Film Array meningitis/encephalitis panel for detection of bacteria, viruses, and yeast in

Chinese children. Ann Transl Med. 2019;7(18):437.