Anti-phospholipase A2 Receptor Antibody Measurement in Patients with Idiopathic Membranous Nephropathy Diagnosed by Renal Biopsy

Sadettin Öztürk; Ozlem Usalan; Celalettin Usalan; Orhan Ozdemir

doi:10.58600/eurjther.20232902-687.y

Authors

Sadettin Öztürk Endocrinology and Metabolism Diseases Clinic, Dr.Ersin Arslan Education Research Hospital, Gaziantep, Turkey https://orcid.org/0000-0002-2992-1511
Ozlem Usalan Department of Internal Medicine, Division of Nephrology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey https://orcid.org/0000-0002-6339-8648
Celalettin Usalan Department of Internal Medicine, Division of Nephrology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey https://orcid.org/0000-0002-9967-1925
Orhan Ozdemir Department of Nephrology, Şanlıurfa Education Research Hospital, Şanlıurfa, Turkey https://orcid.org/0000-0002-9302-1323

DOI:

https://doi.org/10.58600/eurjther.20232902-687.y

Keywords:

membranous nephropathy, anti-PLA2R antibody, proteinuria, anti-phospholipase A2 receptor (anti-PLA2R) antibody, idiopathicmembranous nephropathy (IMN)

Abstract

Objective: Our study is a cross-sectional study that aims to evaluate the presence and levels of anti-phospholipase A2 receptor (PLA2R) antibodies in healthy volunteers and idiopathic membranous nephropathy (IMN) patients and to assess the relationship between these levels and clinical parameters.

Methods: Serum anti-PLA2R antibody levels, complete blood count, urea, creatinine (Kre), total protein,albumin, low-density lipoprotein (LDL)-cholesterol, triglycerides (TG), high-density lipoprotein (HDL)-cholesterol, total cholesterol, C-reactive protein (crp), sedimentation, proteinuria were measured from 71 IMN patients and 48 healthy volunteers.

Results: Of the values compared between the two groups, the urea, creatinine, and modified diet renal disease (MDRD) were similar, total protein, albumin, LDL-cholesterol, TG, total cholesterol, HDL-cholesterol, and complete urinalysis protein values were statistically significantly high in the patient group, as expected in nephrotic syndrome (p<0.01). The anti-PLA2Rantibody levels measured using enzyme-linked immunosorbent assay (ELISA) in patient and control groups were found to be negative. The anti-PLA2R level was found to be 0.104 (0.093-0.129) ng/ml in the IMN group, while it was 0.141 (0.117-0.177) ng/ml in the control group (P=0,001). Although the P value was significant, the anti-PLA2R antibody level was found to be high in the control group and was outside the reference range of the kit.

Conclusion: There is a need to conduct more sensitive studies with a higher number of patients in order to distinguish between primary and secondary nature and to investigate the presence of anti-PLA2R in IMNpatients, which constitute the majority of nephrotic syndromes in adults. Antibody titer levels were observed to be low and it was revealed that the measurement range of the antibody kit used in the study should be more sensitive.

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