Effects of pulsed electromagnetic fields on lipid  peroxidation and antioxidant levels in blood and  liver of diabetic rats: Pulslu elektromanyetik alanın diyabetik ratlarda kan ve karaciğerde  antioksidan düzeylerine ve lipid peroksidasyonuna etkileri

Hafiza Gözen; Can  Demirel; Müslüm Akan; Mehmet  Tarakçıoğlu

doi:10.5152/EurJTher.2017.139

Authors

Hafiza Gözen Department of Physical Therapy and Rehabilitation, Gaziantep University Vocational Higher School
Can Demirel Department of Biophysics, Gaziantep University School of Medicine
Müslüm Akan Department of Biochemistry, Gaziantep University School of Medicine
Mehmet Tarakçıoğlu Department of Biochemistry, Gaziantep University School of Medicine

DOI:

https://doi.org/10.5152/EurJTher.2017.139

Keywords:

Antioxidants, diabetes, free radicals, oxidative stress, pulsed electromagnetic fields

Abstract

Objective: The present study investigated the protective effects of a pulsed electromagnetic field (PEMF) in a rat model of diabetes by analyzing oxidative/nitrosative stress parameters.
Methods: Rats were randomly divided into four groups of eight each: a control group, a sham PEMF group, a diabetes group, and a diabetes+PEMF group. Diabetes was induced in the sham PEMF, diabetes, and diabetes+PEMF groups by treatment with 50 mg/ kg streptozotocin (STZ). Rats in the sham PEMF group were treated identically, without running the instrument. Following the development of diabetes, rats in the diabetes+PEMF group were treated with PEMF for 60 min/day for 4 weeks.
Results: Levels of oxidants, such as malondialdehyde (MDA), nitric oxide (NO), and myeloperoxidase (MPO), and antioxidants, such as superoxide dismutase (SOD), glutathione (GSH), and catalase (CAT), were measured in blood and liver tissue samples. MPO, MDA, and NO levels were significantly higher, and SOD levels significantly lower, in the sham PEMF and diabetes groups than in the control group (p<0.05 each), whereas the levels of all these four (i.e.: MPO, MDA, NO, and SOD) in the diabetes+PEMF group were close to those in the control group. GSH levels were significantly lower in the sham PEMF, diabetes, and diabetes+PEMF groups than in the control group (p<0.05 each), whereas CAT levels were similar in all the four groups.
Conclusion: Results indicate that PEMF affects MDA, NO, MPO, SOD, and GSH levels and regulates diabetes-associated damage by reducing oxidative stress and increasing the levels of antioxidants. PEMF may be a non-invasive treatment option for diabetes and associated complications.

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