Investigation of Brucella and Coxiella burnetii antibodies among humans at risk and control  groups living in southeastern Turkey: Türkiye’nin güneydoğusunda yaşayan risk ve kontrol grubundaki  kişilerde Brucella ve Coxiella burnetii antikorlarının araştırılması

Pınar Arabacı; Fahriye  Ekşi; Ayşen Bayram

doi:10.5152/EurJTher.2017.65

Authors

Pınar Arabacı Department of Medical Microbiology, Gaziantep University School of Medicine
Fahriye Ekşi Department of Medical Microbiology, Gaziantep University School of Medicine
Ayşen Bayram Department of Medical Microbiology, Sanko University School of Medicine

DOI:

https://doi.org/10.5152/EurJTher.2017.65

Keywords:

Brucella spp, Coxiella burnetii, Brucellacapt test, Enzyme-linked immunosorbent assay (ELİSA), mmunofluorescent assay (IFA)

Abstract

Objective: Brucella and Coxiella are zoonotic pathogens with a broad geographic distribution. In this study, we investigated the prevalence of Brucella and Coxiella burnetii (C. burnetii) antibodies among at-risk and control groups living in southeastern Turkey.
Methods: Cross-sectional study. Age, gender, symptoms, and risk factors of subjects were obtained by questionnaire. The presence of Brucella antibodies was determined by Brucellacapt tests. C. burnetii IgM and IgG antibodies were detected by enzyme-linked immunosorbent assay. Positive and equivocal samples were confirmed with an indirect fluorescent-antibody test.
Results: The risk group was composed of farmers (27.7%), butchers (27.3%), laboratory workers (22%), slaughterhouse workers (20%), and veterinarians (3%). The control group was comprised of housewives (36.7%), tradesmen (35%), and office workers (28.3%). For Brucella, 22% of the risk group and 14.7% of the control group had a titer≥1:40 (p=0.020). Of the risk and control groups, 6.7% and 2.7%, respectively, had a titer ≥1:160 (p=0.020). C. burnetii IgM and IgG antibodies were detected in 2% and 40% of the risk group subjects and in 0.7% and 37.3% of the control group subjects, respectively (p=0.285 and p=0.502).
Conclusion: The high prevalence of brucellosis in risk groups compared to the control group and the probability of exposure to C. burnetii, in several sections of the community, especially the risk groups, show the importance of the control of zoonotic diseases.

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