Attenuation of Senescence-Induced Oxidative Exacerbations in Aged Rat Testis by Ferula Elaeochytris Root Extract


Abstract views: 229 / PDF downloads: 0

Authors

DOI:

https://doi.org/10.58600/eurjther-28-4-0118

Keywords:

Ferula elaeochytris, Aging, Antioxidant system, Spermatogenesis

Abstract

Objective: Age brings about changes to the oxidant and antioxidant balance of male testis that give rise to loss of fertility. The Ferula elaeochytris root extract (FE), contents antioxidant and anti-inflammatory component, have been used to treat infertility by local people for centuries. The main objectives of this study were to determine whether FE was effective on sperm quality, spermatogenesis, apoptosis and oxidative stress in aged rat.

Methods: Four groups were formed with 40 rats; young Control (YC), Aged Control (AC), Ferula elaeochytris administrated aged rat (A+FE) and vitamin E administrated aged rat (A+VE). Vitamin E and FE was administered orally for 8 weeks.

Results: The administration of FE significantly increased serum TAS, testetorone levels and decreased testicular malondialdehyde (MDA) activity, that these changes were accompanied by the reduced serum TNF-α, and TOS levels. Also, the apoptosis germ cell, the tubular diameter, the germinal epithelium height and Johnson’s score a have been regulated after administration of FE (p<0.05). Meanwhile, in the present context, in aged group the sperm count, motility, testicular weight declined significantly. FE showed showed significantly increased effect on the motility and sperm count.

Conclusion: These findings support that aging induces stress oxidative and inflammation, and FE could protect the testis against these damaging effects via its anti-oxidative, anti-inflammatory action and modulate spermatogenesis.

Metrics

Metrics Loading ...

References

Mohammadhosseini M, Venditti A, Sarker SD, Nahar L, Akbarzadeh A. The genus Ferula: Ethnobotany, phytochemistry and bioactivities–A review. Industrial crops and products. 2019;129:350-394.

Eser N, Yoldas A. Identification of heat-resistant chemical components of Ferula elaeochytris root extracts by gas chromatographymass spectrometry. Tropical Journal of Pharmaceutical Research. 2019;18(1):55-60.

Eser N, Buyuknacar HS, Cimentepe OO, et al. The effect of Ferula elaeochytris root extract on erectile dysfunction in streptozotocin-induced diabetic rat. International journal of impotence research. 2020;32(2):186-194.

Eser N, Yoldaş A, Yigin A, et al. The protective effect of Ferula elaeochytris on age-related erectile dysfunction. Journal of ethnopharmacology. 2020;258:112921.

Samancioglu A, Sat I, Yildirim E, Ercisli S, Jurikova T, Mlcek J. Total phenolic and vitamin C content and antiradical activity evaluation of traditionally consumed wild edible vegetables from Turkey. 2016.

Aybek H, Aybek Z, Rota S, Şen N, Akbulut M. The effects of diabetes mellitus, age, and vitamin E on testicular oxidative stress. Fertility and sterility. 2008;90(3):755-760.

Lucio RA, Tlachi-López JL, Eguibar JR, Ågmo A. Sperm count and sperm motility decrease in old rats. Physiology & behavior. 2013;110:73-79.

Johnsen SG. Testicular biopsy score count–a method for registration of spermatogenesis in human testes: normal values and results in 335 hypogonadal males. Hormone Research in Paediatrics. 1970;1(1):2-25.

Uchiyamara M, Mihara M. Determination of malondialdehyde in tissue by thiobarbituric acid test. Analytical Biochemistry. 1978;86:271-278.

Zirkin BR, Santulli R, Strandberg JdD Wright WW, Ewing LL. Testicular steroidogenesis in the aging brown Norway rat. Journal of andrology. 1993;14(2):118-123.

Westerterp-Plantenga M. Smeets A, and Lejeune MP. Sensory and gastrointestinal satiety effects of capsaicin on food intake. Int J Obes. 2005;29:682-688.

Vibrans H. Principals and practice of phytotherapy. Modern herbal medicine-Simon Mills, Kerry Bone. Churchill Livingstone, London. 2000. 643+ xx p. ISBN 0-443-060169. Journal of Ethnopharmacology. 2002;1(81):140-141.

Iranshahi M, Alizadeh M. Antihyperglycemic effect of Asafoetida (Ferula assafoetida Oleo-Gum-Resin) in streptozotocin-induced diabetic rats. World Applied Sciences Journal.2012;17(2):157-162.

Patra S, Nithya S, Srinithya B, Meenakshi S. Review of medicinal plants for anti-obesity activity. Translational Biomedicine.2015;6(3).

Deveci E, Tel-çayan G, Duru M, Emin M, Published D. and anti-tyrosinase activities of the various extracts of Ferula elaeochytris and Sideritis stricta. Int J Food Prop. 2018;21:771-783.

Sahoo DK, Roy A. Compromised rat testicular antioxidant defence system by hypothyroidism before puberty. International Journal of Endocrinology. 2012;2012.

Ayuob NN, Al-Harbi MS, Abdulhadi SS. Is the chronic use of Ferula harmonis to enhance mice erectile function effective and safe? A histopathological study. Systems biology in reproductive medicine. 2014;60(5):282-292.

Santiago J, Silva JV, Alves MG, Oliveira PF, Fardilha M. Testicular aging: an overview of ultrastructural, cellular, and molecular alterations. The Journals of Gerontology: Series A. 2019;74(6):860-871.

Mehraein F, Negahdar F. Morphometric evaluation of seminiferous tubules in aged mice testes after melatonin administration Cell Journal (Yakhteh). 2011;13(1):1.

Bruunsgaard H, Skinhøj P, Pedersen AN, Schroll M, Pedersen BK. Ageing, tumour necrosis factor‐alpha (TNF‐α) and atherosclerosis. Clinical & Experimental Immunology. 2000;121(2):255-260.

Wang X, Stocco DM. The decline in testosterone biosynthesis during male aging: a consequence of multiple alterations. Molecular and cellular endocrinology. 2005;238(1-2):1-7.

El-Thaher T, Matalka K, Taha H, Badwan A. Ferula harmonis ‘zallouh’and enhancing erectile function in rats: efficacy and toxicity study. International journal of impotence research. 2001;13(4):247-251.

Osman N. Antioxidant effects of Ferula hermonis and bee honey on γ-radiation-induced oxidative testicular damage in rats. Journal of Radiation Research and Applied Sciences. 2011;4(4A):1201-1219.

Salomon TB, Hackenhaar FS, Almeida AC, et al. Oxidative stress in testis of animals during aging with and without reproductive activity. Experimental gerontology. 2013;48(9):940-946.

Aydın A, Çoban J, Doğan‐Ekici I, Doğru‐Abbasoğlu S, Uysal , Koçak‐Toker N. Carnosine and vitamin E–a promising pair in the combat against testicular oxidative stress in aged rats. Andrologia. 2015;47(10):1131-1138.

Downloads

Published

2022-12-31

How to Cite

Yoldaş, A., Eser, N., Yaylalı, A., Kökbaş, U., & Sarper Bozkurt, A. (2022). Attenuation of Senescence-Induced Oxidative Exacerbations in Aged Rat Testis by Ferula Elaeochytris Root Extract. European Journal of Therapeutics, 28(4), 306–312. https://doi.org/10.58600/eurjther-28-4-0118

Issue

Section

Original Articles