Radioprotective Effects of Propolis and Caffeic acid Phenethyl Ester on the Tongue-Tissues of Total-Head Irradiated Rats

Halis Altay; Elif Demir; Habip Binici; İsmail Aytaç; Muhammed Enes Taysı; Seyithan Taysı

doi:10.5152/eurjther.2020.19047

Authors

Halis Altay Department of Medical Biochemistry, Gaziantep University School of Medicine, Gaziantep https://orcid.org/0000-0001-7878-7884
Elif Demir Division of Biochemistry, Harran University, College of Health, Şanlıurfa https://orcid.org/0000-0003-4545-5175
Habip Binici Clinic of Otolaryngology, Bitlis State Hospital, Bitlis https://orcid.org/0000-0003-4741-4043
İsmail Aytaç Department of Otolaryngology, Gaziantep University School of Medicine, Gaziantep https://orcid.org/0000-0002-0947-366X
Muhammed Enes Taysı Gaziantep University School of Medicine, Gaziantep https://orcid.org/0000-0001-7419-5988
Seyithan Taysı Department of Medical Biochemistry, Gaziantep University School of Medicine, Gaziantep https://orcid.org/0000-0003-1251-3148

DOI:

https://doi.org/10.5152/eurjther.2020.19047

Keywords:

Antioxidants, affeic acid phenethyl ester, irradiation, oxidative stress, propolis

Abstract

Objective: This study aimed to investigate the radioprotective effects of propolis and caffeic acid phenethyl ester (CAPE) against radiation-induced damage in the tongue-tissues of rats exposed to total cranial gamma irradiation.
Methods: Fifty-four Sprague-Dawley rats were randomly divided into six groups. An appropriate control group was also studied. The animals were euthanized on day 10, and tongue-tissues were collected for evaluating biochemical oxidative parameters.
Results: Lipid hydroperoxides (LOOH), total oxidant status (TOS), oxidative stress index (OSI) levels and xanthine oxidase (XO) activity, and markers of oxidative stress were significantly higher, while paraoxonase (PON) activity was significantly lower for irradiated rats (IR) group compared to the other groups. When LOOH, TOS, OSI values, and XO activity in the propolis+IR and CAPE+IR groups were evaluated, there was no statistically significant difference between those ones and all control groups. In terms of the total -SH levels, the propolis+IR group was found to be significantly higher than all other groups. There was no significant difference in arylesterase (ARE) activity, ceruloplasmin (Cp) levels.
Conclusion: Propolis and CAPE reduce oxidative stress and have antioxidant effects that may be useful agents of ionizing radiation-induced tissue damage.

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References

Akyuz M, Taysi S, Baysal E, Demir E, Alkis H, Akan M, et al. Radioprotective effect of thymoquinone on salivary gland of rats exposed to total cranial irradiation. Head Neck 2017; 39: 2027-35.

Taysi S, Tascan AS, Ugur MG, Demir M. Radicals, Oxidative/Nitrosative Stress and Preeclampsia. Mini-Rev Med Chem 2019; 19: 178- 93.

Cikman O, Soylemez O, Ozkan OF, Kiraz HA, Sayar I, Ademoglu S, et al. Antioxidant Activity of Syringic Acid Prevents Oxidative Stress in l-arginine-Induced Acute Pancreatitis: An Experimental Study on Rats. Int Surg 2015; 100:891-6.

Baysal E, Gulsen S, Aytac I, Celenk F, Ensari N, Taysi S, et al. Oxidative stress in otosclerosis. Redox Rep 2017; 22: 235-9.

Ferlay J, Shin HR, Bray F, Forman D, Mathers C, Parkin DM. Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer 2010; 127: 2893-917.

Singh AK, Pandey P, Tewari M, Pandey HP, Gambhir IS, Shukla HS. Free radicals hasten head and neck cancer risk: A study of total oxidant, total antioxidant, DNA damage, and histological grade. J Postgrad Med 2016; 62: 96-101.

Demir E, Taysi S, Al B, Demir T, Okumus S, Saygili O, et al. The effects of Nigella sativa oil, thymoquinone, propolis, and caffeic acid phenethyl ester on radiation-induced cataract. Wien Klin Wochenschr 2016; 128: 587-95.

Khayyo N, Taysi ME, Demir E, Ulusal H, Cinar K, Tarakcioglu M, et al. Radioprotective Effect of Caffeic Acid Phenethyl Ester on the Brain Tissue in Rats Who Underwent Total-Head Irradiation. Eur J Ther2019; 25: 265-72.

Celik E, Taysi S, Sucu S, Ulusal H, Sevincler E, Celik A. Urotensin 2 and Oxidative Stress Levels in Maternal Serum in Pregnancies Complicated by Intrauterine Growth Restriction. Medicina (Kaunas) 2019; 55: 328.

Sezer U, Senyurt SZ, Gundogar H, Erciyas K, Ustun K, Kimyon G, et al. Effect of Chronic Periodontitis on Oxidative Status in Patients with Psoriasis and Psoriatic Arthritis. J Periodontol 2016; 87: 557-65.

Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248-54.

Deniz CD, Aktan M, Erel O, Gurbilek M, Koc M. Evaluation of the radioprotective effects of thymoquinone on dynamic thiol-disulphide homeostasis during total-body irradiation in rats. J Radiat Res 2019; 60: 23-8.

Ahlatci A, Kuzhan A, Taysi S, Demirtas OC, Alkis HE, Tarakcioglu M, et al. Radiation-modifying abilities of Nigella sativa and thymoquinone on radiation-induced nitrosative stress in the brain tissue. Phytomedicine 2014; 21: 740-4.

Hosseinimehr SJ. Trends in the development of radioprotective agents. Drug discovery today 2007; 12: 794-805.

Okumus S, Taysi S, Orkmez M, Saricicek E, Demir E, Adli M, et al. The effects of oral Ginkgo biloba supplementation on radiation-induced oxidative injury in the lens of rat. Pharmacogn Mag 2011; 7: 141-5.

Taysi S, Okumus S, Akyuz M, Uzun N, Aksor A, Demir E, et al. Zinc administration modulates radiation-induced oxidative injury in lens of rat. Pharmacogn Mag 2012; 8: 245-9.

Bacchetti T, Gianna F, Sahebkar A. The role of paraoxonase in cancer. Seminars in Cancer Biology. 2017.

Camps J, Iftimie S, Garcia-Heredia A, Castro A, Joven J. Paraoxonases and infectious diseases. Clin Biochem 2017; 50: 804-11.

Ustun K, Taysi S, Sezer U, Demir E, Baysal E, Demir T, et al. Radio-protective effects of Nigella sativa oil on oxidative stress in tongue-tissue of rats. Oral dis 2014; 20: 109-13.

Zhang C, Peng W, Wang M, Zhu J, Zang Y, Shi W, et al. Studies on protective effects of human paraoxonases 1 and 3 on atherosclerosis in apolipoprotein E knockout mice. Gene Ther 2010; 17: 626-33.

Girotti AW. Lipid hydroperoxide generation, turnover, and effector action in biological systems. J Lipid Res 1998; 39: 1529-42.

Aviram M, Dornfeld L, Rosenblat M, Volkova N, Kaplan M, Coleman R, et al. Pomegranate juice consumption reduces oxidative stress, atherogenic modifications to LDL, and platelet aggregation: studies in humans and in atherosclerotic apolipoprotein E-deficient mice. Am J Clin Nutr 2000; 71: 1062-76.