Evaluation of Diagnostic Performance of Bd Max Ebp Assay in Patients with Diarrheal Illness

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Objective: Detection of the etiological agents in patients with acute diarrhea is challenging due to a wide variety of pathogens. The aim of this study is to evaluate the diagnostic performance of BD Max Enteric Bacterial Pathogens (EBP) PCR assay in patients with diarrheal illness.

Methods: Between 1 January 2014 and 31 May 2015, stool samples from pediatric or adult patients with diarrhea submitted for routine analysis of bacterial stool pathogens were included in the study. We compared the BD Max EBP PCR assay to culture for the detection of Salmonella spp., Shigella spp., Campylobacter jejuni, and Campylobacter coli and an EIA for Shiga toxins 1 and 2. Discordant results were adjudicated by either antigen detection methods or Film array GI Panel.

Results: When coinfections were excluded, the positive percent agreement values for the BD Max EBP assay (PPA) was 100% and negative percent agreement (NPA) was between 98.0%-99.7%, when compared with culture and EIA. After discrepant analysis, the PPA values for the BD Max EBP assay was 100% and NPA was between 99.5%-100%.

Conclusion: The BD Max EBP assay showed a high correlation rate with conventional and molecular methods for the detection of stool pathogens.


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Knabl, L., Grutsch, I., Orth-Höller, D. Comparison of the BD MAX Enteric Bacterial panel assay with conventional diagnostic procedures in diarrheal stool samples. Eur J Clin Microbiol Infect Dis 2016; 35: 131-6.

Anderson, N.W., Buchan, B.W., Ledeboer, N.A.: Comparison of the BD MAX enteric bacterial panel to routine culture methods for detection of Campylobacter, enterohemorrhagic Escherichia coli (O157), Salmonella, and Shigella isolates in preserved stool specimens. J Clin Microbiol 52,1222-4 (2014).

Dryden, M.S., Gabb, R.J., Wright, S.K.: Empirical treatment of severe acute community-acquired gastroenteritis with ciprofloxacin. Clin Infect Dis 1996; 22: 1019-25.

Shane, A.L., Mody, R. K., Crump, J. A., Tarr, P. I., Steiner, T. S., Kotloff, K., et al. Infectious Diseases Society of America Clinical Practice Guidelines for the Diagnosis and Management of Infectious Diarrhea. Clin Infect Dis. 2017; 65: e45–e80.

Guerrant, R.L., Van Gilder, T., Steiner, T.S., Thielman, N.M., Slutsker, L., Tauxe, R.V., Hennessy, T., et al. Infectious Diseases Society of America. Practice guidelines for the management of infectious diarrhea. Clin Infect Dis 2001; 32: 331-51.

Humphries, R.M., Linscott, A.J. Laboratory diagnosis of bacterial gastroenteritis. Clin Microbiol Rev 2015; 28: 3-31.

Operario, D.J., Houpt, E. Defining the causes of diarrhea: novel approaches. Curr Opin Infect Dis 2011; 24: 464-71.

Ozyurt, O. K., Bertocco, A.L.F.V.B., Pereira, L.A.B., Jimenes, L.P., Yazisiz, H., Ozhak, B., et al. Detection of Salmonella, Campylobacter,

Shiga toxin-producing E. coli and Shigella/EIEC by culture and a multiplex PCR panel in pediatric patients with acute diarrheal illness. Journal of Laboratory Medicine 2019;43: 211-5.

Harrington, S.M., Buchan, B.W., Doern, C., Fader, R., Ferraro, M.J., Pillai, D.R., et al. Multicenter evaluation of the BD max enteric bacterial panel PCR assay for rapid detection of Salmonella spp., Shigella spp., Campylobacter spp. (C. jejuni and C. coli), and Shiga toxin 1 and 2 genes. J Clin Microbiol 2016; 54: 1251-8.

Riddle, M.S., DuPont, H.L., Connor, B.A. ACG Clinical Guideline: Diagnosis, Treatment, and Prevention of Acute Diarrheal Infections in Adults. Am J Gastroenterol 2016; 111: 602-22.

Biswas, J.S., Al-Ali, A., Rajput, P., Smith, D., Goldenberg, S.D. A paralel diagnostic accuracy study of three molecular panels for the detection of bacterial gastroenteritis. Eur J Clin Microbiol Infect Dis 2014; 33: 2075–2081.

Mortensen, J.E., Ventrola, C., Hanna, S. Comparison of time-motion analysis of conventional stool culture and the BD MAX™ Enteric Bacterial Panel (EBP). BMC Clin Pathol 2015;15: 9.




How to Cite

Koyuncu Ozyurt, O., Saglik, I., Ozhak, B., Mutlu, D., Levent, B., Donmez, L., Ongut, G., Colak, D., & Ogunc, D. (2022). Evaluation of Diagnostic Performance of Bd Max Ebp Assay in Patients with Diarrheal Illness. European Journal of Therapeutics, 28(4), 297–300. https://doi.org/10.58600/eurjther-28-4-0098



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