Investigation of Various Virulence Factors and  SCCmec Types in the Healthcare-associated  and Community-associated Methicillin Resistant  Staphylococcus aureus Strains

Süreyya Gül Yurtsever; Abdurrahman Aygül; İsmail Öztürk; Salih Atakan Nemli; Selçuk Kaya; Şafak Ermertcan

doi:10.5152/eurjther.2019.19062

Authors

Süreyya Gül Yurtsever Department of Medical Microbiology, İzmir Katip Çelebi University School of Medicine, İzmir
Abdurrahman Aygül Department of Pharmaceutical Microbiology, Çukurova University Faculty of Pharmacy, Adana
İsmail Öztürk Department of Pharmaceutical Microbiology, İzmir Katip Çelebi University Faculty of Pharmacy, İzmir
Salih Atakan Nemli Department of Infectious Diseases, İzmir Katip Çelebi University School of Medicine, İzmir
Selçuk Kaya Department of Medical Microbiology, İzmir Katip Çelebi University School of Medicine, İzmir
Şafak Ermertcan Department of Pharmaceutical Microbiology, Ege University Faculty of Pharmacy, İzmir

DOI:

https://doi.org/10.5152/eurjther.2019.19062

Keywords:

Methicillin-resistant Staphylococcus aureus, PCR typing, SCCmec, virulence factors

Abstract

Objective: The objective of this study was to investigate some virulence genes and SCCmec types of methicillin-resistant Staphylococcus aureus (MRSA) isolates and to determine their relationship with virulence factors.
Methods: A total of 100 MRSA strains, 64 from healthcare-associated and 36 from community-associated infections, were included in the study. The presence of mecA gene was investigated by PCR. SCCmec types and efb, clfB, agrA gene were detected by multiplex PCR and their relationship with virulence factors has been analysed.
Results: All of the isolates contain the mecA gene. At the same time, in 66 strains (66%) agrA gene, in 58 strains (58%) clfB gene, and in 47 strains (47%) efb gene were positive. In terms of SCCmec types, the distribution of these types among the 64 HA-SA strains was 53% similar-to-type-III, 16% type IV, 2% type I and 30% unclassified. The distribution of the types among the 36 CA-SA strains was 19% similar-to-type-III, 25% type IV, 8% type I and 47% unclassified, respectively. When SCCmec types were evaluated according to clinical sample type, similar-to-type-III isolates were found to be dominant in wound samples. Efb (78%), clfB (85%), agrA (88%) were the dominant genes in similar-to-type-III strains, whereas clfB (74%), agrA (100%) were the main genes detected in the type IV strains.
Conclusion: It is of clinical and epidemiological importance to know the origin of MRSA strains because this affects the empirical treatment choice.

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