Dynamic Thiol/ Disulfide Balance in Children  with Acute Malnutrition

Mahmut Demir; Özcan Erel

doi:10.5152/eurjther.2019.19040

Authors

Mahmut Demir Department of Pediatrics, Harran University School of Medicine, Şanlıurfa https://orcid.org/0000-0002-0983-9457
Özcan Erel Department of Biochemistry, Yıldırım Beyazıt University School of Medicine, Ankara https://orcid.org/0000-0002-2996-3236

DOI:

https://doi.org/10.5152/eurjther.2019.19040

Keywords:

Children, malnutrition, oxidative stress, thiol/ disulfide balance

Abstract

Objective: In the human body, a balance exists between the production of free radicals and suppression of increased levels of reactive oxygen species by the antioxidant system. If this balance is disturbed, oxidative stress occurs. Free radicals produced during normal metabolism or pathological processes affect the structure and functions of thiol-dependent enzymes and the thiol/disulfide balance in the cell environment. The aim of our study was to evaluate the dynamic thiol/disulfide balance in children with acute malnutrition.
Methods: Fifty-two patients diagnosed with acute malnutrition according to Waterlow classification and 40 healthy children were included in the study. The weight, weight Z score, height, height Z score, and BMI of the patients in the study group were measured. The thiol/disulfide balance was measured in both groups using the automatic method developed by Erel and Neselioğlu.
Results: There was no statistically significant difference between the patient and control groups in terms of average native thiol and total thiol levels. The disulfide levels and the disulfide/native thiol and disulfide/total thiol ratios were significantly higher in the patient group than in the control group, whereas the native thiol/total thiol ratio was significantly lower in the patient group than in the control group.
Conclusion: Our findings indicate that the thiol/disulfide balance is weakened in children with acute malnutrition and is shifted toward disulfide.

Metrics

Metrics Loading ...

References

World Health Organization. Indicators for assessing infant and young child feding practices: Part III Country Profiles. WHO, Geneva, 2010.

Gómez F, Ramos Galvan R, Frenk S, Cravioto Muñoz J, Chávez R, Vázquez J. Mortality in second and third degree malnutrition. Bull World Health Organ 2000; 78: 1275-80.

McCarthy H, Dixon M, Crabtree I, Eaton-Evans MJ, McNulty H. The development and evaluation of the screening tool for the assessment of malnutrition in paediatrics (STAMP(©) ) for use by health care staff. J Hum NutrDiet 2012; 25: 311-8.

Ozcan Erel. A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem 2004; 37: 112-9.

Ozcan Erel. A new automated colorimetric method for measuring total oxidant status. Clin Biochem 2005; 38: 1103-11.

Erel O, Neselioglu S. A novel and automated assay for thiol/ disulfide balance. ClinBiochem 2014; 47: 326-32.

Oliveira PVS, Laurindo FRM. Implications of plasma thiol redox in disease. Clin Sci (Lond) 2018 Jun 21; 132: 1257-80.

Erenler AK, Yardan T. Clinical utility of thiol/ disulfide homeostasis. Clin Lab 2017 May 1; 63: 867-70.

Neyzi O, Yalcindag A, Alp H. Heights and weights of Turkish children. J Trop Pediatr Environ Child Health 1973; 19: 5-13.

Waterlow JC. Note on the assessment and classification of protein-energy malnutrition in children. Lancet 1973; 2: 87-9.

Kumar S, Olson DL, Schwenk WF. Part I. Malnutrition in the pediatric population. DisMon 2002; 48(11): 703-12.

Sezer K, Keskin M. Role of the free oxygen radicals on the pathogenesis of the diseases. Fırat University Veterinary Journal of Health Sciences 2014; 28: 49-56.

Ece A, Gürkan F, Celik F, Boşnak M, Yel S, Balik H, et al. Paraoxonase, total antioxidant activity and peroxide levels in marasmic children: relationships with leptin. Clin Biochem 2007; 40: 634-9.

Celik M, Sermatov K, Abuhandan M, Zeyrek D, Kocyigit A, Iscan A. Oxidative status and DNA damage in chidren with marasmic malnutrition. J Clin Lab Anal 2012; 26: 161-6.

Circu ML, Aw TY. Reactive oxygen species, cellular redox systems, and apoptosis. Free Radic Biol Med 2010 Mar 15; 48: 749-62.

Uttara B, Singh AV, Zamboni P, Mahajan, RT. Oxidative stres and neurodegenerative diseases: a review of upstream and down stream antioxidant therapeutic options. Curr Neuropharmacol 2009; 7: 65-74.

Üstüner P. Thiol/ disulfide homeostasis as a novel inflammatory marker in vitiligo. Medical Journal of Istanbul Kanuni Sultan 2018; 10: 18-24.

Ates I, Altay M, Yilmaz FM, Topcuoglu C, Neselioglu S, Erel O, et al. Dynamic thiol/ disulfide balance in patients with autoimmune subclinical hypothyroidism. Endocr Res 2016; 41: 343-9.

Durmuş SY, Şahin NM, Ergin M, Neşelioğlu S, Aycan Z, Erel Ö. How does thiol/ disulfide homeostasis change in children with type 1 diabetes mellitus? Diabetes research and clinical practice 2019; 149: 64-8.

Ozdamar K, Sen A, Koyuncu I. The use of the thiol-disulphide homeostasis as an indicator of oxidative stress in pediatric adenoid hypertrophy patients. SANAMED 2019; 14: 37-43.

Incecik F, Avcıoğlu G, Erel Ö, Neşelioğlu S, Hergüner OM. Dynamic thiol/ disulfide homeostasis in children with Duchenne muscular dystrophy. Acta Neurol Belg 2019; 119: 215-8.