The Effects of Some Phytotherapeutic Plants on Escherichia coli spp. that are Exposed to Different Doses of Gamma Radiation

Mehmet Cihan Yavaş; Mustafa Salih Çelik

doi:10.5152/EurJTher.2019.18060

Authors

Mehmet Cihan Yavaş Department of Biophysics, Kırşehir Ahi Evran University School of Medicine, Kırşehir https://orcid.org/0000-0002-2923-050X
Mustafa Salih Çelik Department of Nursing, Batman University School of Health, Batman https://orcid.org/0000-0001-7397-8120

DOI:

https://doi.org/10.5152/EurJTher.2019.18060

Keywords:

Antioxidant, Escherichia coli spp., gamma radiation, phytotherapeutic plants, plant extract

Abstract

which gamma radiation at different doses was applied. By this way, the effects of antioxidant ethanolic extracts of plants and the effect of gamma radiation on Escherichia coli spp. (E. coli) were investigated.
Methods: In the present study, E. coli spp. in the areas of agar and agar+plant extracts (83 µl) was irradiated by increasing gamma radiation (100, 200, 500, 1000, 3000, and 6000 cGy). In our study, six plants (carob (Ceratonia siliqua L.), basil (Ocimum basilicum L.), ginger (Zingiber officinale), rosemary (Rosmarinus officinalis L.), yarrow (Achillea millefolium L.), and cumin (Cuminum cyminum L.) were found to be consumed by people, and their phytotherapeutic effects were investigated.
Results: In the present study, a decrease in the number of colonies of E. coli spp. was demonstrated due to the increasing gamma radiation dose. It has been assumed that the ethyl alcohol components of phytotherapeutic plants cannot be assessed but are distributed and clustered on the Petri dish and plant extracts may show antioxidant effects.
Conclusion: It is understood that some phytotherapeutic plants used in gamma radiation applications may show a protective effect.

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References

Mu H, Sun J, Li L, Yin J, Hu N, Zhao W, et al. Ionizing radiation exposure: hazards, prevention, and biomarker screening. Env Sci Poll Res 2018; 25: 15294-306.

Stewart RD. Induction of DNA damage by light ions relative to 60Co ϒ-rays. Int J Particle Ther 2018; 5: 25-39.

Soghomonyan D, Margaryan A, Trchounian K, Ohanyan K, Budalyan H, Trchounian A. The effects of low doses of gamma-radiation on growth and membrane activity of Pseudomones aeruginosa GRP3 and Escherichia coli M17. Cell Biochem Biophys 2018; 76: 209-217.

Kamiya K, Ozasa K, Akiba S, Niwa O, Kodama K, Takamura N, et al. Long-term effects of radiation exposure on health. Lancet 2015; 386: 469-78.

Maier RM, Pepper IL. Environment microbiology, Chapter 3, Academic Press, 37-56; 2014.

Çelik MS. Determination of the number of bacteria in liquid medium by spectrophotometric method. Dicle Med J 1983; 10: 97-104.

Tungmunnithum D, Thongboonyou A, Pholboon A, Yangsabai A. Flavonoids and other phenolic compounds from medicinal plants for pharmaceutical and medical aspects: an overview. Medicines 2018; 5: 93.

Ribeiro-Santos R, Carvalho-Costa D, Cavalerio C, Costa HS, Gonçalves Albuquerque T, Conceição Castilho M, et al. A novel insight on a ancient aromatic plant: the rosemary (Rosmarinus offinalis L.). Trends Food Sci Technol 2015; 45: 355-68

Akimoto M, Lizuka M, Kanematsu R, Yoshida M, Takenaga K. Anticancer effect of Ginger extract against pancreatic cancer cells mainly through reactive oxygen species-mediated autotic cell death. PloS One 2015; 10: e0126605.

Verma AK, Kakani RK, Solanki RK, Meena RD. Improvement in yield attributing traits of cumin (Cuminum cyminum) through acute exposure of gamma ray. Int J Pure App Biosci 2017; 5: 312-8.

El Shalat AN, El Shennawy HM, Abd El Megid MHM. Studying the protective effect of gamma-irradiated basil (Ocimum basilicum L.) against methotrexate-induced liver and renal toxicity in rat. Indian J Anim Res 2017; 51: 135-40.

Mahtout R, Zaidi F, Saadi LO, Boudjou S, Oomah BD, Hosseinian F. Carob (Ceratonia siliqua L.) supplementation affects kefir quality and antioxidant capacity during storage. Int J Eng Tech 2016; 2: 168- 77.

Applequist WL, Moerman DE. Yarrow (Achillea millefolium L.): A neglected panacea? A review of ethnobotany, bioactivity, and biomedical research. Economic Botany 2011; 65: 209-25.

Zamaro LL, Pêrez-Gracia MT. Using digital photography to implement the McFarland method. J R Soc Interface 2012; 9: 1892-7.

Wu W, Linu SA, Ogony J, Matthews R, Goldstein GA, Ercal N. Effects of N- acetylcysteine amide ( NACA), a thiol antioxidant on radiation-induced cytotoxicity in chinese hamster ovary cell. Life Sci 2008; 82: 1122-30.

Phaniendra A, Jestadi DB, Periyasamy L. Free radicals: properties, sources, targets, and their implication in various diseases. Indian J Clin Biochem 2015; 30: 11-26.

Islam MT, da Mata AM, de Aguiar RP, Paz MF, de Alencar MV, Ferreira PM, et al. Therapeutic potential of essential oils focusing on diterpenes. Phytother Res 2016; 30: 1420-44.

Fathiazad F, Matlobi A, Khorrami A, Hamedeyazdan S, Soraya H, Hammami M, et al. Phytochemical screening and evaluation of cardioprotective activity of ethanolic extract of Ocimum basilicum L. (basil) against isoproterenol induced myocardial infarction in rats. Daru 2012; 20: 87.

Petiwala SM, Johnson JJ. Diterpenes from rosemary (Rosmarinus officinalis): Defining their potential for anti-cancer activity. Cancer Lett 2015; 367: 93-102.

Si W, Chen YP, Zhang J, Chen ZY, Chung HY. Antioxidant activities of ginger extract and its constituents toward lipids. Food Chem 2018; 239: 1117-25.

Moghaddam M, Miran SNK, Pirbalouti AG, Mehdizadeh L, Ghaderi Y. Variation in essential oil composition and antioxidant activity of cumin (Cuminum cyminum L.) fruits during stages of maturity. Ind Crops Prod 2015; 70: 163-9.

Navaie BA, Kavoosian S, Fattahi S, Hajian-Tilaki K, Asouri M, Bishekolaie R, et al. Antioxidant and cytotoxic effect of aqueous and hydroalcoholic extracts of the Achillea millefolium L. on MCF-7 breast cancer cell line. Int Biol Biomed J 2015; 1: 119-25.

Tai J, Cheung S, Wu M, Hasman D. Antiproliferation effect of Rosemary (Rosmarinus officinalis) on human ovarian cancer cells in vitro. Phytomedicine 2012; 19: 436-43.