The Effect of Smoking on Gingival Crevicular Fluid Sclerostin and TNF-Α Levels in Patient with Periodontitis

Mehmet Oğuzhan Ergin; Kamile Erciyas; Mehmet Tarakçıoğlu; hasan ulusal

doi:10.58600/eurjther2370

Authors

Mehmet Oğuzhan Ergin Department of Periodontology, Faculty of Dentistry, Harran University, Şanlıurfa, Türkiye https://orcid.org/0000-0003-3216-9698
Kamile Erciyas Department of Periodontology, Faculty of Dentistry, Gaziantep University, Gaziantep, Türkiye https://orcid.org/0000-0001-9940-0423
Mehmet Tarakçıoğlu Department of of Medical Biochemistry, Faculty of Medicine, Gaziantep University, Gaziantep, Türkiye https://orcid.org/0000-0002-2398-8264
Hasan Ulusal Department of of Medical Biochemistry, Faculty of Medicine, Gaziantep University, Gaziantep, Türkiye https://orcid.org/0000-0003-3890-2088

DOI:

https://doi.org/10.58600/eurjther2370

Keywords:

Gingival fluid, , Periodontitis, , Smoking, Sklerostin, TNF-α

Abstract

Objective: The objective of this study was to evaluate the impact of cigarette smoking on sclerostin and TNF-α levels in subjects exhibiting periodontally healthy conditions and those afflicted with periodontitis. The hypothesis tested was that sclerostin levels in smokers could serve as a diagnostic marker and a tool to assess the progression of the disease.

Methods: In the present study, gingival crevicular fluid samples were obtained from a total of 72 patients, who were divided into four distinct groups: a control group of 18 non-smoking individuals without periodontitis, a group of 18 non-smoking individuals with periodontitis, a group of 18 healthy individuals who smoked, and a group of 18 smokers with periodontitis. The levels of sclerostin and TNF-α were then evaluated using the enzyme-linked immunosorbent assay (ELISA) method.

Results: The findings indicated a positive correlation between sclerostin and TNF-α levels in the gingival fluid of individuals diagnosed with periodontal disease.Subsequent evaluations of the relationship between smoking and these biomarkers revealed a statistically significant increase in TNF-α levels, while the increase in sclerostin levels did not attain statistical significance. However, when the analyses were performed without taking into account age-related effects within groups (adjusted for age), a statistically significant increase in sclerostin levels due to smoking was observed.

Conclusion: In conclusion, it can be posited that the measurement of TNF-α levels in smokers may serve as a biomarker for the diagnosis and progression of the disease. Further studies are required to determine the role of sclerostin in this context.

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References

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