Effect of Vitamin K2 on Blood Rheology and Vascular Responses in Diabetic Rats

Serra Ozturk Bostancı; Nur Özen; Ege Alkan; Özge Güzelad; Hande Salım; Ahmet Yıldırım; Pınar Ülker; Filiz Basralı; Deniz Erbaş; Muzaffer Sindel

doi:10.58600/eurjther2214

Authors

Serra Ozturk Bostancı Department of Anatomy, Faculty of Medicine, Akdeniz University, Antalya, Türkiye https://orcid.org/0000-0003-1002-0059
Nur Özen Department of Basic Medical Sciences, Faculty of Dentistry, Antalya Bilim University, Antalya, Türkiye https://orcid.org/0000-0002-2332-3449
Ege Alkan Department of Anatomy, Faculty of Medicine, Akdeniz University, Antalya, Türkiye https://orcid.org/0000-0002-7805-5808
Özge Güzelad Department of Anatomy, Faculty of Medicine, Akdeniz University, Antalya, Türkiye https://orcid.org/0000-0003-0327-4886
Hande Salım Department of Anatomy, Faculty of Medicine, Hitit University, Çorum, Türkiye https://orcid.org/0000-0002-7231-9526
Ahmet Yıldırım Department of Physiology, Faculty of Medicine, Akdeniz University, Antalya, Türkiye https://orcid.org/0000-0001-5930-9756
Pınar Ülker Department of Physiology, Faculty of Medicine, Akdeniz University, Antalya, Türkiye https://orcid.org/0000-0003-1772-5641
Filiz Basralı Department of Physiology, Faculty of Medicine, Akdeniz University, Antalya, Türkiye https://orcid.org/0000-0002-6692-7779
Deniz Erbaş Department of Physiology, Faculty of Medicine, University of Kyrenia, Northern, Cyprus https://orcid.org/0000-0002-1468-4146
Muzaffer Sindel Department of Anatomy, Faculty of Medicine, Akdeniz University, Antalya, Türkiye https://orcid.org/0000-0002-6594-1325

DOI:

https://doi.org/10.58600/eurjther2214

Keywords:

Diabetes Mellitus, Vitamin K2, Menaquinone-7, Endothelial dysfunction

Abstract

Background/aim: Diabetes is manifested by endothelial dysfunction and an imbalance between vasoconstriction and vasodilation. The aim of our study is to examine the effect of vitK2 application on vascular and rheological parameters in a rat diabetes model.

Materials and methods: A total of 60 male Wistar Albino rats were used to examine vascular responses and hemorheological parameters. A total of 6 groups were: control (C), control+vehicle (Cv), control+vitK2 administered (C + K2), diabetes (D), diabetes+vehicle (Dv), and diabetes+vitK2 (D + K2) group. After the animals were sacrificed, blood and vascular samples were taken and the contraction and relaxation responses of the aorta and erythrocyte deformability and aggregation were examined.

Results: When KCl dose-response curves are evaluated; Increased vasoconstriction response was found in the Dv group compared to the Cv group. The increase in vasoconstriction observed in the Dv group decreased with the application of vit K2. D+vitK2 group thoracic aorta contraction responses returned to Cv group levels. In response to increasing cumulative doses of Phe, a significant increase in vasoconstriction response was observed in the Dv group compared to the Cv group. VitK2 application reduced the Phe-mediated contractile response, which was increased in the Dv group, and returned the contraction response to Cv conditions except for two intermediate Phe doses. In the Dv + K2 group, a significant decrease was observed in the aggregation index, which was tended to increase.

Conclusion: Considering the cardiovascular complications frequently observed in diabetes, it can be suggested that vitK2 therapy may yield positive outcomes in diabetes.

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