Effects of Exposure to Radiofrequency at 2.45 GHz on Structural Changes Associated with Lipid Peroxidation in Prepubertal Rat Testicular Tissue
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DOI:
https://doi.org/10.58600/eurjther1875Keywords:
Testicular Injury, Prepubertal Rats, Malondialdehyde, Glutathione, RadiofrequencyAbstract
Objective: The increasing use of electronic devices, accompanied by advancing technologies, has led to heightened exposure to non-ionizing electromagnetic radiation (EMR). This exposure instigates the accumulation of free radicals and oxidative damage in tissues, consequently impacting biological systems. Notably, the testis is among the tissues adversely affected by EMR. Numerous studies have highlighted the pivotal role of the testis in sperm production, emphasizing the potential implications of any damage on the reproductive system. This study aims to assess the levels of lipid peroxidation through histological evaluation in the testicular tissue of prepubertal male rats exposed to electromagnetic radiation at varying electric field intensities within the 2.45 GHz radiofrequency (RF) range.
Methods: The experimental group comprises six subdivisions, including a sham control group, as well as groups exposed to varying electric field strengths (EFS) of 0.6 V/m, 1.9 V/m, 5 V/m, 10 V/m, and 15 V/m, respectively. Excluding the sham control group, the remaining subgroups were subjected to a daily 2.45 GHz RF exposure for 1 hour starting immediately after fertilization. This exposure to different electric field intensities continued for 45 days post-birth.
Results: The samples obtained from the RF radiation-exposed rats exhibited elevated malondialdehyde (MDA) values and decreased glutathione (GSH) values in the testicular tissue. Furthermore, a comparative analysis between the microwave radiation-exposed group and the control group revealed distinct histological alterations in the testicular tissue.
Conclusion: In conclusion, our findings indicate that exposure to microwave radiation at an electric field intensity of 15 V/m can lead to significant histopathological and oxidative parameter changes in Wistar rats. These results underscore the potential effects of such exposure on human health.
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Feychting M, Ahlbom A (1993) Magnetic fields and cancer in children residing near Swedish high-voltage power lines. Am J Epidemiol. 138(7):467-81. https://doi.org/10.1093/oxfordjournals.aje.a116881
Moon JH (2020) Health effects of electromagnetic fields on children. Clin Exp Pediatr. 63(11):422-428. https://doi.org/10.3345/cep.2019.01494
Watson R (2011) Radiation fears prompt possible restrictions on wi-fi and mobile phone use in schools. Bmj. 342:d3428. https://doi.org/10.1136/bmj.d3428
Laudisi F, Sambucci M, Nasta F, Pinto R, Lodato R, Altavista P, Lovisolo GA, Marino C, Pioli C (2012) Prenatal exposure to radiofrequencies: effects of WiFi signals on thymocyte development and peripheral T cell compartment in an animal model. Bioelectromagnetics. 33(8):652-61. https://doi.org/10.1002/bem.21733
Ozgur E, Kismali G, Guler G, Akcay A, Ozkurt G, Sel T, Seyhan N (2013) Effects of prenatal and postnatal exposure to GSM-like radiofrequency on blood chemistry and oxidative stress in infant rabbits, an experimental study. Cell Biochem Biophys. 67(2):743-51. https://doi.org/10.1007/s12013-013-9564-1
Keshvari J, Keshvari R, Lang S (2006) The effect of increase in dielectric values on specific absorption rate (SAR) in eye and head tissues following 900, 1800 and 2450 MHz radio frequency (RF) exposure. Phys Med Biol. 51(6):1463-77. https://doi.org/10.1088/0031-9155/51/6/007
Almášiová V, Holovská K, Andrašková S, Cigánková V, Ševčíková Z, Raček A, Andrejčáková Z, Beňová K, Tóth Š, Tvrdá E, Molnár J, Račeková E (2021) Potential influence of prenatal 2.45 GHz radiofrequency electromagnetic field exposure on Wistar albino rat testis. Histol Histopathol. 36(6):685-696. https://doi.org/10.14670/hh-18-331
Dietert RR, Piepenbrink MS (2008) The managed immune system: protecting the womb to delay the tomb. Hum Exp Toxicol. 27(2):129-34. https://doi.org/10.1177/0960327108090753
Aldad TS, Gan G, Gao XB, Taylor HS (2012) Fetal radiofrequency radiation exposure from 800-1900 mhz-rated cellular telephones affects neurodevelopment and behavior in mice. Sci Rep. 2:312. https://doi.org/10.1038/srep00312
Balassa T, Varró P, Elek S, Drozdovszky O, Szemerszky R, Világi I, Bárdos G (2013) Changes in synaptic efficacy in rat brain slices following extremely low-frequency magnetic field exposure at embryonic and early postnatal age. Int J Dev Neurosci. 31(8):724-30. https://doi.org/10.1016/j.ijdevneu
Hancı H, Odacı E, Kaya H, Aliyazıcıoğlu Y, Turan İ, Demir S, Çolakoğlu S (2013) The effect of prenatal exposure to 900-MHz electromagnetic field on the 21-old-day rat testicle. Reprod Toxicol. 42:203-9. https://doi.org/10.1016/j.reprotox.2013.09.006
Zhang Y, Li Z, Gao Y, Zhang C (2015) Effects of fetal microwave radiation exposure on offspring behavior in mice. J Radiat Res. 56(2):261-8. https://doi.org/10.1093/jrr/rru097
Othman H, Ammari M, Rtibi K, Bensaid N, Sakly M, Abdelmelek H (2017) Postnatal development and behavior effects of in-utero exposure of rats to radiofrequency waves emitted from conventional WiFi devices. Environ Toxicol Pharmacol. 52:239-247. https://doi.org/10.1016/j.etap.2017.04.016
Guler G, Tomruk A, Ozgur E, Seyhan N (2010) The effect of radiofrequency radiation on DNA and lipid damage in non-pregnant and pregnant rabbits and their newborns. Gen Physiol Biophys. 29(1):59-66. https://doi.org/10.4149/gpb_2010_01_59
D'Silva MH, Swer RT, Anbalagan J, Rajesh B (2017) Effect of Radiofrequency Radiation Emitted from 2G and 3G Cell Phone on Developing Liver of Chick Embryo - A Comparative Study. J Clin Diagn Res. 11(7):Ac05-ac09. https://doi.org/10.7860/jcdr/2017/26360.10275
Sharma A, Kesari KK, Saxena VK, Sisodia R (2017) The influence of prenatal 10 GHz microwave radiation exposure on a developing mice brain. Gen Physiol Biophys. 36(1):41-51. https://doi.org/10.4149/gpb_2016026
Kilcoyne KR, Mitchell RT (2019) Effect of environmental and pharmaceutical exposures on fetal testis development and function: a systematic review of human experimental data. Hum Reprod Update. 25(4):397-421. eng. Epub 2019/03/15. https://doi.org/10.1093/humupd/dmz004
Fejes I, Závaczki Z, Szöllosi J, Koloszár S, Daru J, Kovács L, Pál A (2005) Is there a relationship between cell phone use and semen quality? Arch Androl. 51(5):385-93. https://doi.org/10.1080/014850190924520
Agarwal A, Deepinder F, Sharma RK, Ranga G, Li J (2008) Effect of cell phone usage on semen analysis in men attending infertility clinic: an observational study. Fertil Steril. 89(1):124-8. https://doi.org/10.1016/j.fertnstert.2007.01.166
Shokri S, Soltani A, Kazemi M, Sardari D, Mofrad FB (2015) Effects of Wi-Fi (2.45 GHz) Exposure on Apoptosis, Sperm Parameters and Testicular Histomorphometry in Rats: A Time Course Study. Cell J. 17(2):322–331. https://doi.org/10.22074/cellj.2016.3740
Mantiply ED, Pohl KR, Poppell SW, Murphy JA (1997) Summary of measured radiofrequency electric and magnetic fields (10 kHz to 30 GHz) in the general and work environment. Bioelectromagnetics. 18(8):563–577.
Joseph W, Verloock L, Goeminne F, Vermeeren G, Martens L (2012) Assessment of RF exposures from emerging wireless communication technologies in different environments. Health Phys. 102(2):161–172. https://doi.org/10.1097/HP.0b013e31822f8e39
Nassiri P, Esmaeilpour MR, Gharachahi E, Haghighat G, Yunesian M, Zaredar N (2013) Exposure assessment of extremely low frequency electric fields in Tehran, Iran, 2010. Health Phys. 104(1):87–94. https://doi.org/10.1097/HP.0b013e31826f51c1
Mihara M, Uchiyama M (1978) Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem. 86(1):271-8. https://doi.org/10.1016/0003-2697(78)90342-1
Sedlak J, Lindsay RH (1968) Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem. 25(1):192-205. https://doi.org/10.1016/0003-2697(68)90092-4
Izzati SN, Armalina D, Ariani RMD, Saktini F (2021) Histopathology of Seminiferous Tubules of Wistar Rats (Rattus norvegicus) Exposed to Electric Mosquito Repellent with Active Ingredient D-Allethrin, Jurnal Kedokteran Diponegoro (Diponegoro Medical Journal). 10(5):390-395. https://doi.org/10.14710/dmj.v10i5.31588
Johnsen SG. Testicular biopsy score count--a method for registration of spermatogenesis in human testes: normal values and results in 335 hypogonadal males. Hormones. https://doi.org/10.1159/000178170
Marino C, Lagroye I, Scarfì MR, Sienkiewicz Z (2011) Are the young more sensitive than adults to the effects of radiofrequency fields? An examination of relevant data from cellular and animal studies. Prog Biophys Mol Biol. 107(3):374-85. https://doi.org/10.1016/j.pbiomolbio.2011.09.002
Kismali G, Ozgur E, Guler G, Akcay A, Sel T, Seyhan N (2012) The influence of 1800 MHz GSM-like signals on blood chemistry and oxidative stress in non-pregnant and pregnant rabbits. Int J Radiat Biol. 88(5):414-9. https://doi.org/10.3109/09553002.2012.661517
Balci M, Devrim E, Durak I (2007) Effects of mobile phones on oxidant/antioxidant balance in cornea and lens of rats. Curr Eye Res. 32(1):21-5. https://doi.org/10.1080/02713680601114948
Kumar S, Kesari KK, Behari J (2010) Evaluation of genotoxic effects in male Wistar rats following microwave exposure. Indian J Exp Biol. 48(6):586-92. https://doi.org/10.3109/09553000903564059
Meral I, Mert H, Mert N, Deger Y, Yoruk I, Yetkin A, Keskin S (2007) Effects of 900-MHz electromagnetic field emitted from cellular phone on brain oxidative stress and some vitamin levels of guinea pigs. Brain Res. 1169:120-4. https://doi.org/10.1016/j.brainres.2007.07.015
Rao VS, Titushkin IA, Moros EG, Pickard WF, Thatte HS, Cho MR (2008) Nonthermal effects of radiofrequency-field exposure on calcium dynamics in stem cell-derived neuronal cells: elucidation of calcium pathways. Radiat Res. 169(3):319-29. https://doi.org/10.1667/rr1118.1
Kumar S, Nirala JP, Behari J, Paulraj R (2014) Effect of electromagnetic irradiation produced by 3G mobile phone on male rat reproductive system in a simulated scenario. Indian J Exp Biol. 52(9):890-7. https://doi.org/10.1111/and.13201
Meena R, Kumari K, Kumar J, Rajamani P, Verma HN, Kesari KK (2014) Therapeutic approaches of melatonin in microwave radiations-induced oxidative stress-mediated toxicity on male fertility pattern of Wistar rats. Electromagn Biol Med. 33(2):81-91. https://doi.org/10.3109/15368378.2013.781035
Saunders RD, Kowalczuk CI (1981) Effects of 2.45 GHz microwave radiation and heat on mouse spermatogenic epithelium. Int J Radiat Biol Relat Stud Phys Chem Med. 40(6):623-32. https://doi.org/10.1080/09553008114551611
Dasdag S, Ketani MA, Akdag Z, Ersay AR, Sari I, Demirtas OC, Celik MS (1999) Whole-body microwave exposure emitted by cellular phones and testicular function of rats. Urol Res. 27(3):219-23. https://doi.org/10.1007/s002400050113
Ozguner M, Koyu A, Cesur G, Ural M, Ozguner F, Gokcimen A, Delibas N (2005) Biological and morphological effects on the reproductive organ of rats after exposure to electromagnetic field. Saudi Med J. 26(3):405-10. https://doi.org/10.1002/jat.1584
Salama N, Kishimoto T, Kanayama HO (2010) Effects of exposure to a mobile phone on testicular function and structure in adult rabbit. Int J Androl. 33(1):88-94. https://doi.org/10.1111/j.1365-2605.2008.00940.x
Hasan I, Amin T, Alam MR, Islam MR (2021) Hematobiochemical and histopathological alterations of kidney and testis due to exposure of 4G cell phone radiation in mice. Saudi J Biol Sci. 28(5):2933-2942. https://doi.org/10.1016/j.sjbs.2021.02.028
Alkis ME, Akdag MZ, Dasdag S, Yegin K, Akpolat V (2019) Single-strand DNA breaks and oxidative changes in rat testes exposed to radiofrequency radiation emitted from cellular phones. Biotechnology & Biotechnological Equipment. 33(1):1733-1740. https://doi.org/10.1080/13102818.2019.1696702
Yurekli AI, Ozkan M, Kalkan T, Saybasili H, Tuncel H, Atukeren P, Gumustas K, Seker S (2006) GSM base station electromagnetic radiation and oxidative stress in rats. Electromagn Biol Med. 25(3):177-88. https://doi.org/10.1080/15368370600875042
Kesari KK, Kumar S, Behari J (2011) Effects of radiofrequency electromagnetic wave exposure from cellular phones on the reproductive pattern in male Wistar rats. Appl Biochem Biotechnol. 164(4):546-59. https://doi.org/10.1007/s12010-010-9156-0
Ceyhan AM, Akkaya VB, Güleçol Ş C, Ceyhan BM, Özgüner F, Chen W (2012) Protective effects of β-glucan against oxidative injury induced by 2.45-GHz electromagnetic radiation in the skin tissue of rats. Arch Dermatol Res. 304(7):521-7. https://doi.org/10.1007/s00403-012-1205-9
Oyewopo AO, Olaniyi SK, Oyewopo CI, Jimoh AT (2017) Radiofrequency electromagnetic radiation from cell phone causes defective testicular function in male Wistar rats. Andrologia. 49(10). https://doi.org/10.1111/and.12772
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