Plasma Leptin, Nesfatin 1, NPY, and Zinc Levels in Obese and Metabolic Syndrome Children

Adnan Aydogdu; Omer Unal; Saltuk Bugra Baltaci; Esma Menevse; Rasim Mogulkoc; Said Sami Erdem; Muammer Buyukinan; Abdulkerim Kasim Baltaci

doi:10.58600/eurjther1760

Authors

Adnan Aydogdu Departments of Physiology and Biochemistry and Pediatric Endocrinology, Selçuk University, Faculty of Medicine, Konya, Turkey https://orcid.org/0000-0002-1825-0097
Omer Unal Department of Physiology, Faculty of Medicine, Kirikkale University, Kirikkale, Turkey https://orcid.org/0000-0002-4816-830X
Saltuk Bugra Baltaci Department of Physiology, Regenerative and Restorative Medical Research Center, Istanbul Medipol University, Istanbul, Turkey https://orcid.org/0000-0002-9752-7508
Esma Menevse Departments of Physiology and Biochemistry and Pediatric Endocrinology, Selçuk University, Faculty of Medicine, Konya, Turkey https://orcid.org/0000-0002-5477-5667
Rasim Mogulkoc Department of Physiology, Faculty of Medicine, Selçuk University, Konya, Türkiye https://orcid.org/0000-0001-6155-6780
Said Sami Erdem Department of Biochemistry and Pediatric Endocrinology, Konya Training and Research Hospital, Konya, Turkey https://orcid.org/0000-0002-1011-7240
Muammer Buyukinan Faculty of Medicine, Departments of Physiology and Biochemistry and Pediatric Endocrinology, Selçuk University, Konya, Turkey https://orcid.org/0000-0002-2937-823X
Abdulkerim Kasim Baltaci Departments of Physiology and Biochemistry and Pediatric Endocrinology, Selçuk University, Faculty of Medicine, Konya, Turkey https://orcid.org/0000-0003-2461-1212

DOI:

https://doi.org/10.58600/eurjther1760

Keywords:

Children, metabolic syndrome, Obesity, NPY, leptin, nesfatin-1, zinc

Abstract

Objective: The aim of this study is to investigate the relationship between leptin, nesfatin 1 and NPY hormones and zinc in boys and girls diagnosed with metabolic syndrome and obesity.

Materials and Methods: This study included a total of 6 groups. Group 1 Boy Control, Group 2 Girl Control, Group 3 Obese Boys, Group 4 Obese Girls, Group 5 Boys with Metabolic Syndrome, Group 2 with Girls with Metabolic Syndrome. Plasma leptin, nesfatin-1, NPY (by ELISA method) and serum zinc (by AA method) levels were determined in blood samples obtained from the subjects.

Results: Leptin and zinc levels were significantly higher both in boy and girl patients with metabolic syndrome than in obese and control children. Nesfatin-1 and NPY levels were significantly lower both in girl and boy obese and metabolic syndrome children compared to their control groups.

Conclusion: In the current study a significant increase in plasma leptin and serum zinc levels and a significant decrease of plasma nesfatin-1 and NPY levels were observed in boys and girls with metabolic syndrome. The findings of our study show that leptin, nesfatin-1 and NPY levels may be important biomarkers in the assessment of metabolic syndrome risk in both girls and boys.

Metrics

Metrics Loading ...

References

Bellanger TM, Bray GA (2005) Obesity related morbidity and mortality. J La State Med Soc. 157(1): 42-49.

Agrawal A, Prakash YS (2014) Obesity, Metabolic Syndrome, and Airway Disease: A Bioenergetic Problem? Immunol Allergy Clin North Am. 34(4):785-796. https://doi:10.1016/j.iac.2014.07.004

Ziylan YZ, Baltaci AK, Mogulkoc R (2009) Leptin transport in the central nervous system. Cell Biochem Funct. 27(2):63-70. https://doi:10.1002/cbf.1538

Yosten GL, Samson WK (2009) Nesfatin-1 exerts cardiovascular actions in brain: possible interaction with the central melanocortin system. Am J Physiol Regul Integr Comp Physiol. 297(2):330-336. https://doi:10.1152/ajpregu.90867.2008

Kohno D, Yada T (2012) Arcuate NPY neurons sense and integrate peripheral metabolic signals to control feeding. Neuropeptides. 46(6):315-319. https://doi:10.1016/j.npep.2012.09.004

Baltaci AK, Mogulkoc R (2012) Leptin and zinc relation: In regulation of food intake and immunity. Indian J Endocrinol Metab. 16:611-616. https://doi:10.4103/2230-8210.105579

Selvais PL, Labuche C, Nguyen XN, Ketelslegers JM, Denef JF, Maiter DM (1997) Cyclic feeding behaviour and changes in hypothalamic galanin and neuropeptide Y gene expression induced by zinc deficiency in the rat. J Neuroendocrinol. 9(1):55-62. https://doi:10.1046/j.1365-2826.1997.00566.x

Bundak R, Furman A, Gunoz H, Darendeliler F, Bas F, Neyzi O (2006) Body mass index references for Turkish Children. Acta Paediatr. 95(2):194-198. https://doi:10.1080/08035250500334738

Hatipoglu N, Ozturk A, Mazicioglu MM, Kurtoglu S, Seyhan S, Lokoglu F (2008) Waist circumference percentiles for 7-17 year old Turkish children and adolescents. Eur J Pediatr. 167(4):383-389. https://doi:10.1007/s00431-007-0502-3

Valle M, Martos R, Gascón F, Cañete R, Zafra MA, Morales R (2005) Low Low grade systemic inflammation, hypoadiponectinemia and a high concentration of leptin are present in very obese children and correlate with metabolic syndrome. Diabetes Metab. 31(1):55-62. https://doi:10.1016/s1262-3636(07)70167-2

Madeira I, Bordallo MA, Rodrigues NC, Carvalho C, Gazolla F, Collett-Solberg P, Medeiros C, Bordallo AP, Borges M, Monteiro C, Ribeiro R (2017) Leptin as a predictor of metabolic syndrome in prepubertal children. Arch Endocrinol Metab. 61(1):7-13. https://doi:10.1590/2359-3997000000199

Obeidat AA, Ahmad MN, Haddad FH, Azzeh FS (2016) Leptin and uric acid as predictors of metabolic syndrome in jordanian adults. Nutr Res Pract. 10(4):411-417. https://doi:10.4162/nrp.2016.10.4.411

Alp E, Görmüş U, Güdücü N, Bozkurt S (2015) Nesfatin-1 levels and metabolic markers in polycystic ovary syndrome. Gynecol Endocrinol. 31(7):543-547. https://doi:10.3109/09513590.2015.1024219

Algul S, Ozkan Y, Ozcelik O (2016) Serum nesfatin-1 levels in patients with different glucose tolerance levels. Physiol Res. 65(6):979-985. https://doi:10.33549/physiolres.933186

Aksu O, Aydın B, Doguç DK, Ilhan I, Ozturk O, Altuntas A, Demirkan H, Koroglu BK, Tamer MN (2015) The evaluation of Nesfatin-1 levels in patients with OSAS associated with metabolic syndrome. J Endocrinol Invest 38(4):463-469. https://doi:10.1007/s40618-014-0216-0

Nakata M, Yada T (2013) Role of NUCB2/nesfatin-1 in glucose control: diverse functions in islets, adipocytes and brain. Curr Pharm Des. 19(39):6960-6965. https://doi:10.2174/138161281939131127130112

Ailanen L, Ruohonen ST Vähätalo L, Tuomainen K, Eerola K, Salomäki-Myftari H, Röyttä M, Laiho A, Ahotupa M, Gylling H, Savontaus E (2017) The metabolic syndrome in mice overexpressing neuropeptide Y in noradrenergic neurons. J Endocrinol. 234(1):57-72. https://doi:10.1530/JOE-16-0223

Rabaglino MB, Moreira-Espinoza MJ, Lopez JP, Garcia NH, Beltramo D (2016) Maternal Triclosan consumption alters the appetite regulatory network on Wistar rat offspring and predispose to metabolic syndrome in the adulthood. Endocr J. 63(11):1007-1016. https://doi:10.1507/endocrj.EJ16-0257

Lemche E, Chaban OS, Lemche AV (2016) Neuroendorine and epigentic mechanisms subserving autonomic imbalance and HPA dysfunction in the Metabolic Syndrome. Front Neurosci. 10:142. https://doi:10.3389/fnins.2016.00142

Vähätalo LH, Ruohonen ST, Mäkelä S, Ailanen L, Penttinen AM, Stormi T, Kauko T, Piscitelli F, Silvestri C, Savontaus E, Di Marzo V (2015) Role of the endocannabinoid system in obesity induced by neuropeptide Y overexpression in noradrenergic neurons. Nutr Diabetes. 5(4):e151. https://doi:10.1038/nutd.2015.1

Gunes M, Bukan N (2015) Examination of angiopoietin-like protein 4, neuropeptide Y, omentin-1 levels of obese and non-obese patients with polycystic ovary syndrome. Gynecol Endocrinol. 31(11):903-906.i https://doi:10.3109/09513590.2015.1068285

Jang M, Mistry A, Swick AG, Romsos DR (2000) Leptin rapidly inhibits hypothalamic neuropeptide Y secretion and stimulates corticotropin-releasing hormone secretion in adrenalectomized mice. J Nutr 130(11):2813-2820. https://doi:10.1093/jn/130.11.2813

Baltaci AK, Mogulkoc R, Baltaci SB (2019) Review: The role of zinc in the endocrine system. Pak J Pharm Sci. 32(1):231-239.

Demirci Ş, Gün C (2022) Zinc Supplementation Improved Neuropeptide Y, Nesfatin-1, Leptin, C-reactive protein, and HOMA-IR of Diet-Induced Obese Rats. Biol Trace Elem Res. 200(9):3996-4006. https://doi:10.1007/s12011-021-02987-6

Seo JA, Song SW, Han K, Lee KJ, Kim HN (2014) The associations between serum zinc levels and metabolic syndrome in the Korean population: findings from the 2010 Korean National Health and Nutrition Examination Survey. PLoS One 9(8):e105990. https://doi:10.1371/journal.pone.0105990

Hosseini-Esfahani F, Mirmiran P, Koochakpoor G, Daneshpour MS, Guity K, Azizi F (2017) Some dietary factors can modulate the effect of the zinc transporters 8 polymorphism on the risk of metabolic syndrome. Sci Rep 7:1649. https://doi:10.1038/s41598-017-01762-9

Mazloomi S, Alizadeh N, Aminzare M, Niroomand S, Mousavi SN (2017) Serum zinc and adiponectin levels in patients with polycystic ovary syndrome, adjusted for anthropometric, biochemical, dietary intake, and physical activity measures. Biol Trace Elem Res. 181(2):388. https://doi:10.1007/s12011-017-0951-0

Torkanlou K, Bibak B, Abbaspour A, Abdi H, Saleh Moghaddam M, Tayefi M, Mohammadzadeh E, Safarian Bana H, Aghasizade M, Ferns GA, Avan A,Ghayour Mobarhan M (2016) Reduced serum levels of zinc and superoxide dismutase in obese individuals. Ann Nutr Metab. 69(3-4):232-236. https://doi:10.1159/000454894

Magge SN, Goodman E, Armstrong SC (2017) The Metabolic syndrome in children and adolescents: Shifting the focus to cardiometabolic risk factor Clustering. Pediatrics 140(2): e20171603. https://doi:10.1542/peds.2017-1603

Jansen J, Karges W, Rink L (2009) Zinc and diabetes--clinical links and molecular mechanisms. J Nutr Biochem. 20(6):399-417. https://doi:10.1016/j.jnutbio.2009.01.009

Jansen J, Rosenkranz E, Overbeck S, Warmuth S, Mocchegiani E, Giacconi R, Weiskirchen R, Karges W, Rink L (2012) Disturbed zinc homeostasis in diabetic patients by in vitro and in vivo analysis of insulinomimetic activity of zinc. J Nutr Biochem. 23(11):1458-1466. https://doi:10.1016/j.jnutbio.2011.09.008

Yu Y, Cai Z, Zheng J, Chen J, Zhang X, Huang XF, Li D (2012) Serum levels of polyunsaturated fatty acids are low in Chinese men with metabolic syndrome, whereas serum levels of saturated fatty acids, zinc, and magnesium are high. Nutr Res. 32(2):71-77. https://doi:10.1016/j.nutres.2011.12.004