Dietary Zinc Status in Offspring of Pregnant Rats Fed on a Zinc-Deficient Diet Is Associated with Serum Albumin, Ast, and Alt Levels

Abstract views: 79 / PDF downloads: 73




Maternal zinc deficiency, zinc, Albumin, AST, ALT, male offspring rat


Objective: This study was carried out to investigate whether dietary zinc status is associated with serum albumin, AST and ALT levels in male offspring of mother rats fed a zinc deficient diet.

Methods: The study was carried out on male offspring (Groups 1, 2, 3) born to rats fed a zinc deficient diet and on male offspring (Group 4) born to mothers fed a standard diet. Group 1: Zinc deficient, Group 2; standard rat chow, Group 3: Zinc supplemented diet. Animals of group 4 were used as control group. After the completion of the experimental stages of the study, albumin, AST, ALT, free and total bilirubin levels in serum samples taken from animals were determined by spectrophotometric method.

Results: Dietary zinc deficiency (group 1) significantly decreased serum albumin values (p<0.004). Animals in both the zinc deficient (Group 1) group and the Group 2 animals born to mothers fed a zinc deficient diet and fed standard rat chow had the highest AST and ALT levels (p<0.001).

Conclusion: The present study is the first to show that dietary zinc status can directly affect liver function in rats born to zinc deficient mothers by causing changes in serum albumin, AST and ALT levels.


Metrics Loading ...


Bloom A, Bloom S, Silva H, Nicoll AJ, Sawhney R (2021) Zinc supplementation and its benefits in the management of chronic liver disease: An in-depth literature review. Ann Hepatol. 100549.

Riggio O, Merli M, Capocaccia L, Caschera M, Zullo A, Pinto G, Gaudio E, Franchitto A, Spagnoli R, D'Aquilino E (1992) Zinc supplementation reduces blood ammonia and increases liver ornithine transcarbamylase activity in experimental cirrhosis. Hepatology 16(3):785-789.

Vikbladh I (1951) Studies on zinc in blood II. Scand J Clin Lab Invest. 3 (Suppl. (2):1-74.

Ebara M, Fukuda H, Hatano R, Yoshikawa M, Sugiura N, Saisho H, Kondo F, Yukawa M (2003) Metal contents in the liver of patients with chronic liver disease caused by hepatitis C virus. Reference to hepatocellular carcinoma. Oncology 65 (4):323-330.

Omran DA, Darweesh SK, Fouad H, Mahmoud M, Saif S, Fared A, Hassany M, Mobarak L, El-Tahawy MA, Yosry A (2017) Serum zinc deficiency and its relation to liver fibrosis in chronic HCV: a real-life Egyptian study. Biol Trace Elem Res. 179 (1):1-7.

Sengupta S, Wroblewski K, Aronsohn A, Reau N, Reddy KG, Jensen D, Te H (2015) Screening for zinc deficiency in patients with cirrhosis: When should we start? Dig Dis Sci. 60(10):3130-3135

Tan HK, Streeter A, Cramp ME, Dhanda AD (2020) Effect of zinc treatment on clinical outcomes in patients with liver cirrhosis: A systematic review and meta-analysis. World J Hepatol. 12(7):389-398.

Grüngreiff K, Gottstein T, Reinhold D, Blindauer CA (2021) Albumin substitution in decompensated liver cirrhosis: Don't forget zinc. Nutrients. 13(11):4011.

Grüngreiff K, Reinhold D, Wedemeyer H (2016) The role of zinc in liver cirrhosis.Ann Hepatol. 15(1):7-16.

Llibre-Nieto G, Lira A, Vergara M, Solé C, Casas M, Puig-Diví V, Solé G, Humanes A, Grau L, Barradas JM, Miquel M, Sánchez-Delgado J (2021) Micronutrient deficiencies in patients with decompensated liver cirrhosis. Nutrients. 13(4):1249.

Himoto T, Masaki T (2018) Associations between zinc deficiency and metabolic abnormalities in patients with chronic liver disease. Nutrients. 10(1):88.

Celik D, Tatar B, Köse S, Odemis I (2020) Evaluation of the diagnostic validity of noninvasive tests for predicting liver fibrosis stage in chronic hepatitis B patients. Acta Gastroenterol Belg. 83(3):419-425.

Dalgıç B (2002) Çocuklarda Karaciğer Hastalıklarının Değerlendirilmesi. STED 11(5):173-176.

Goktepe E, Baltaci SB, Unal O, Unlukal N, Mogulkoc R, Baltaci AK (2023) The relationship between beta cell activation and SLC30A8/ZnT8 levels of the endocrine pancreas and maternal zinc deficiency in rats. J Trace Elem Med Biol. 2023 May 19;79:127217.

Baltaci AK, Mogulkoc R, Baltaci SB (2019) Review: The role of zinc in the endocrine system. Pak J Pharm Sci. 32(1):231-239.

Katayama K (2020) Zinc and protein metabolism in chronic liver diseases. Nutr Res. 74:1–9.

Yang W, Wang X, Yu Z, Li C, Sun M, Li Y, Hui Y, Guo G, Fan X, Jiang K, Sun C (2022) Low levels of serum zinc associate with malnutrition risk assessed by the Royal Free Hospital-Nutritional Prioritizing tool in cirrhosis. Biol Trace Elem Res. 200(10):4289-4296.

Hedegaard CV, Soerensen MD, Jørgensen LH, Schaffalitzky de Muckadell OB (2022) Investigating hypozincemia and validity of plasma zinc measurements in infected patients. Scand J Clin Lab Invest. 82(5):371-377.

Morisaku M, Ito K, Ogiso A, Imai M, Hiraoka Y, Zennami M, Tsuge M, Mori M, Toyosato S, Matsuda H, Ando Y, Tokuda M, Tomita A, Yamada S (2022) Correlation between serum albumin and serum zinc in malignant lymphoma. Fujita Med J. 8(2):59-64.

Wang S, Fan X, Gao Y, Zuo L, Hong M, Xu Y (2023) The relationship between zinc deficiency and hepatocellular carcinoma associated with Hepatitis B liver cirrhosis: A 10-year follow-up study. Biol Trace Elem Res. 201(1):114-120.

Sidhu P, Garg ML, Dhawan DK (2005) Time dependent study to evaluate the efficacy of zinc on hepatic marker enzymes and elemental profile in serum and liver of protein deficient rats. Biometals. 18(1):97-106.

Yousef MI, El-Hendy HA, El-Demerdash FM, Elagamy EI (2002) Dietary zinc deficiency induced-changes in the activity of enzymes and the levels of free radicals, lipids and protein electrophoretic behavior in growing rats. Toxicology 175(1-3):223-234.

Centers for Disease Control and Prevention (CDC) (2013) Notes from the field: Zinc deficiency dermatitis in cholestatic extremely premature infants after a nationwide shortage of injectable zinc - Washington, DC, December 2012. MMWR Morb Mortal Wkly Rep. 62(7):136-137.




How to Cite

Gümüş, M., & Baltaci, S. B. (2023). Dietary Zinc Status in Offspring of Pregnant Rats Fed on a Zinc-Deficient Diet Is Associated with Serum Albumin, Ast, and Alt Levels. European Journal of Therapeutics, 573–578.

Funding data