Prevention of reperfusion induced lung injury by L-carnitine

M. Cumhur Sivrikoz; Eser Öz; Velit Halit; Aykut Altunkaya; Haşim Üstünsoy; Işık Şenkaya; Yıldırım İmren; Candan Özoğul

doi:10.58600/eurjther.2001-12-1-1465-arch

Authors

M. Cumhur Sivrikoz Gaziantep Üniversitesi Tıp Fakültesi Göğüs Kalp ve Damar Cerrahisi Anabilim Dalı, Gaziantep
Eser Öz Gazi Üniversitesi Tıp Fakültesi Fizyoloji Anabilim Dalı, Ankara
Velit Halit Gazi Üniversitesi Tıp Fakültesi Göğüs Kalp Damar Cerrahisi Anabilim Dalı, Ankara
Aykut Altunkaya Gazi Üniversitesi Tıp Fakültesi Göğüs Kalp Damar Cerrahisi Anabilim Dalı, Ankara
Haşim Üstünsoy Gaziantep Üniversitesi Tıp Fakültesi Göğüs Kalp ve Damar Cerrahisi Anabilim Dalı, Gaziantep
Işık Şenkaya Uludağ Üniversitesi Tıp Fakültesi Göğüs Kalp ve Damar Cerrahisi Anabilim Dalı, Bursa
Yıldırım İmren Gazi Üniversitesi Tıp Fakültesi Göğüs Kalp Damar Cerrahisi Anabilim Dalı, Ankara
Candan Özoğul Gazi Üniversitesi Tıp Fakültesi Histoloji ve Embriyoloji Anabilim Dalı, Ankara

DOI:

https://doi.org/10.58600/eurjther.2001-12-1-1465-arch

Keywords:

L-Carnitine, Lung, Ischemia-reperfusion injury

Abstract

L-Carnitine plays an important role in the transmembrane transport of long chain fatty ascids for it's oxidation and supresses the development of free radical damage. An experimental comparative study isolated guinea pig lungs has been undertaken to determine the probable beneficial effects of adding L-Carnitine to preischemic pulmonary perfusion solutions in lung ischemia-reperfusion. The isolated lungs (n=10 in control group) previously being perfused by oxygenated Krebs Henseleit solution were put in normothermic ischemic conditions. 1 O mmol of L-Carnitine was added into a Krebs-Henseleit solution in the experimental group (n= 10) and after 20 min., perfusion was completed and the lungs were then exposed to normothermic ischemia. After 3 hours of normothermic ischemia, the lungs were perfused with Krebs-Henseleit solution for 20 minutes. Tissue malondialdehyde (MDA), glutathione (GSH) levels and electron microscopic evaluations were held on before the ischemic period and also at the end of reperfusion. MDA and GSH levels of the perfusate were measured before ischemic period and at the end of reperfusion. L-Carnitine administration reduced MDA levels both in tissues and perfusates. in contrast to glutathione levels which is increased. According to our data, the addition of L Carnitine to preischemic pulmonary perfusion solution showed a significant protective effect regarding both ischemic and reperfusion injury.

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