Significance of p53, bcl-2 and Fas oncogenes in the Iaryngeal squamous celi carcinoma

Yıldırım A. Bayazıt; Kemal Bakır; Ramazan Uçak; Enver Özer; Semih Mumbuç; Muzaffer Kanlıkama

doi:10.58600/eurjther.2001-12-1-1421-arch

Authors

Yıldırım A. Bayazıt Department of Otolaryngology Head and Neck Surgery, Gaziantep University, Gaziantep, Turkey
Kemal Bakır Department of Pathology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey
Ramazan Uçak 2Department of Pathology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey
Enver Özer Department of Otolaryngology Head and Neck Surgery, Gaziantep University, Gaziantep, Turkey
Semih Mumbuç of Pathology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey
Muzaffer Kanlıkama Department of Otolaryngology Head and Neck Surgery, Gaziantep University, Gaziantep, Turkey

DOI:

https://doi.org/10.58600/eurjther.2001-12-1-1421-arch

Keywords:

squamous cell laryngeal carcinoma, fas, bcl-2, oncoprotein, squamous cell laryngeal carcinoma

Abstract

Objective: To assess whether the expression of oncoproteins, p53, bcl-2 and fas, correlated with the clinical and pathological parameters. in squamous celi laryngeal carcinoma (SCLC).

Study design: Medical records of 30 patients who were operated for SCLC, were reviewed, and histopathological and immunhistochemical assessments of the corresponding tissue samples were performed.

Methods: The monoclonal antibodies to p53 bcl-2 and fas proteins were used for immunhistochemical staining. Streptavidin-Biotin, horseradish method was used. The data obtained was compared with the clinical and histopathological results.

Results: A positive staining for p53 was obtained in 19 (63.3% ) of 30 tumors. Only one (3.3%) of 30 tumors positively stained for bcl-2. A positive staining for fas was obtained in 27 (90%) of the tumors. The results of p53 and bcl-2 did not correlate with mitotic count (MC), grade, T and N, and smoking. There was a correlation between fas and MC (p=0.007). But, the results of fas did not correlate with grade, T, N and smoking. A correlation was not found between; p53 vs.bcl-2 (p<0.05); p53 vs. fas (p<0.05); and bcl-2 vs. fas (p<0.05).

Conclusion: There was no association between the oncoproteins (p53, bcl-2 and fas) and clinical or pathological parameters studied. The expression of the oncoproteins between smokers and non smokers did not show signifıcant difference as well.

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