The Possible Role of 3':5'- Cyclic Guanosine Monophosphate in Acetylcholine-Mediated Concraction of Isolated Rat Duodenum

Aydın ERENMEMİŞOĞLU; Cem SÜER; Sadun TEMOÇİN; Hüseyin BEYDAĞI

doi:10.58600/eurjther.1994-5-1-1369-arch

Authors

Aydın ERENMEMİŞOĞLU Erciyes Üniversitesi Tıp Fakültesi Farmakoloji ABD.Yrd.Doç.Dr.
Cem SÜER Erciyes Üniversitesi Tıp Fakültesi Fizyoloji ABD.Doç.Dr.
Sadun TEMOÇİN Çukurova Üniversitesi Tıp Fakültesi Fizyoloji ABD.Yrd.Doç.Dr.
Hüseyin BEYDAĞI Gaziantep Üniversitesi Tıp Fakültesi Fizyoloji ABD.Yrd.Doç.Dr.

DOI:

https://doi.org/10.58600/eurjther.1994-5-1-1369-arch

Keywords:

Acetylcholine, 3':5'-cyclic guanosine monophosphate, sodium nitroprusside, methylene blue

Abstract

This study was planned to determine whether the possible role of 3':5'-cyclic guanosine monophosphate (cylic GMP) on acetylcholine(ACh) action in isolated rat duodenum. Therefore, sodium nitroprusside(SNP) and methylene blue(MB) were used as a guanylate cyclase activator and inhibitor respectively. MB caused significant fall in ACh-induced contraction peaks, whereas SNP did not alter them. These results suggested that cyclic GMP may play a role for ACh action in isolated duodenum smooth muscle. However further experiments are necessary to determine whether a causal relationship exists between cyclic GMP and ACh action.

Metrics

Metrics Loading ...

References

lgnarro LJ., Burke TM., Woods KS., et all.:Assodation between cyclic GMP accumulation and acetylcholine-elicited relaxation of bovine intrapulmonary artery. J Pharmacol Exp Ther 228:682-690, 1984.

Bolton TB.:Mechanisms of action of transmitters and other subtances on smooth muscle. Physiol Rev 59:607-718, 1979.

Furchgott RF., Zawadziki JV.:The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine. Nature(Lond.) 288:373-376, 1980.

Schultz KD., Schiltz K., Schultz G.:Sodium nitroprusside and other smooth muscle relaxants increase cyclic GMP levels in rat ductus deferens. Nature (lond) 288:750-751, 1979.

Fujitani Y., Ueda H., Okada T., Urade Y., Kraki H.:A selective agonist of endothelin type B receptor, IRL 1620, stimulates cyclic GMP increase via nitric oxide formation in rat aorta. J Pharm Exp Ther 267:683-689, 1993.

Süer C., Erenmemişoğlu A., Temoçin S., Akar S., Beydağı H., Taspolat l.:lnfluence of calcium removal from bath solution on isolated rat duodenum contractions. Indian J Physiol Pharmacol 36:181-184, 1992.

Ross EM.,1990 Pharmacodynamics:Mechanism of drug action and the relationship between drug concentration and effect. In:The Pharmacological Basis of Therapeutics.(8 th ed) Gilman AG, Rall TW, Nies AS, Taylor P Eds. New York, Pergamon Press; 1990:166-186.

Lee T., Kuo JF.:Role of muscarinic cholinergic receptors in regulation of guanosine 3':5'-cyclic monophosphate content in mammalian brain, hearth muscle and intestinal smooth muscle, Proc Natl Acad Sci(USA). 69:3287-3291, 1972.