Agents Isolated from Vaginal Cultures in the Reproductive Period and Their Antibiotic Sensitivities (Vaginal Culture and Antibiotic Sensitivitie)


Abstract views: 71 / PDF downloads: 26

Authors

DOI:

https://doi.org/10.58600/eurjther-131

Keywords:

vaginal discharge, vaginitis, antibiotic sensitivity

Abstract

Introduction: In our study, we aimed to examine the strains isolated from vaginal swab samples sent to our laboratory from various clinics with a pre-diagnosis of vulvovaginitis and antibiotic resistance rates,  retrospectively.

Methods: ethods: 90 vaginal swab samples of patientswere included into this study. Two samples were taken from each patient and one sample was used for Gram staining. The other sample was inoculated in 5% sheep blood, Eozine Methylene Blue agar and Saboraud dextrose agar. TIdentification of isolated colonies and antibiotic susceptibility studies were carried out with Vitek 2 Compact automated system. Result of the susceptibility testing are reported according to EUCAST criteria. Nugent scoring was used for bacterial vaginosis.

Results: Normal vaginal flora elements were found in 66 (73.3 %) of the specimens, and 28 organisms were found in 24 (26.7 %). The distribution of the organisms is as follows: 6 (21.4%) Escherichia coli, 5(17.9%) Streptococcus agalactiae,  5 (17.9%) Gardnerella vaginalis, 3 (10.7%) Candida spp., 3(10.7%) Klebsiella pneumoniae, 3(10.7%) Enterococcus faecalis. Two of the E.coli strains and one of the K.pneumoniae strains are ESBL positive. Of the agents, 21 were isolated from outpatients and 7 from inpatients.  All S. agalactiae strains were susceptible to penicillin and ampicillin. E.coli and K.pneumoniae strains, which are the most frequently isolated Gram (-) organisms, are most susceptible to amikacin, carbapenems and tigecycline.

Conclusion: In patients with vaginal discharge and itching complaints, determining the organisms with microbiological culture and oriented treatment instead of empirical treatment will be more beneficial for cure.

Metrics

Metrics Loading ...

References

- FNeal CM, Kus LH, Eckert LO, Peipert JF. Noncandidal vagi-nitis: a comprehensive approach to diagnosis and manage-ment. Am J Obstet Gynecol. 2020;222(2):114-122.

- Hillier SL, Austin M, Macio I, Meyn LA, Badway D, Beigi

R. Diagnosis and Treatment of Vaginal Discharge Syn-dromes in Community Practice Settings. Clin Infect Dis. 2021;72(9):1538-1543.

- Oliveira AS, Rolo J, Gaspar C, Palmeira de Oliveira R, Marti-nez de Oliveira J, Palmeira de Oliveira A. Allergic vulvovag-initis: a systematic literature review. Arch Gynecol Obstet. 2022;306(3):593-622

- Mills BB. Vaginitis: Beyond the Basics. Obstet Gynecol Clin North Am. 2017;44(2):159-177.

- American College of Obstetricians and Gynecologists’ Com-mittee on Practice Bulletins—Gynecology. Diagnosis and Management of Vulvar Skin Disorders: ACOG Practice Bulletin, Number 224. Obstet Gynecol. 2020;136(1):e1-e14.

- Nenadić D, Pavlović MD. Value of bacterial culture of vaginal swabs in diagnosis of vaginal infections. Vojnosanit Pregl. 2015;72(6):523-528.

- EUCAST. European Committee on Antimicrobial Susceptibil-ity Testing Breakpoint tables for interpretation of MICs and zone diameters European Committee on Antimicrobial Sus-ceptibility Testing Breakpoint ts for interpretation of MICs and zone diameters. Eur Comm Antimicrob Susceptibility Testing Break tables Interpret MICs Zo diameters Version 80, 2018 http//www.eucast.org.2015;0-77.

- Powell AM, Nyirjesy P. Recurrent vulvovaginitis. Best Pract Res Clin Obstet Gynaecol 2014;28(7):967–976.

- Diop K, Dufour JC, Levasseur A, Fenollar F. Exhaustive rep-ertoire of human vaginal microbiota. Human Microbiome Journal 2019;11:1-7.

- Green KA, Zarek SM, Catherino WH. Gynecologic health and disease in relation to the microbiome of the female repro-ductive tract. Fertil Steril. 2015;104(6):1351–1357.

- Lopes dos Santos Santiago G, Tency I, et al. Longitudinal qPCR study of the dynamics of L. crispatus, L. iners, A. vagi-nae, (Sialidase Positive) G. vaginalis, and P. bivia in the vagi-na. PLoS One 2012; 7(9)

- Baek JC, Jo HC, Lee SM, Park JE, Cho IA, Sung JH. Prevalence of Pathogens and Other Microorganisms in Premenopaus-al and Postmenopausal Women with Vulvovaginal Symp-toms: A Retrospective Study in a Single Institute in South Korea. Medicina (Kaunas). 2021;57(6):577.

- Machado A, Almeida C, Salgueiro D, et al. Fluorescence in situ hybridization method using Peptide Nucleic Acid probes for rapid detection of Lactobacillus and Gardnerella spp. BMC Microbiol 2013;13(1).

- Kadir MA, Sulyman MA, Dawood IS, Shams-Eldin S. Tricho-monas vaginalis and associated microorganisms in wom-en with vaginal discharge in Kerkuk-Iraq. Ankara Med J 2014;14(3):91–9.

- Bolukaoto JY, Monyama CM, Chukwu MO, Lekala SM, Nch-abeleng M, Maloba MRB, vd. Antibiotic resistance of Strep-tococcus agalactiae isolated from pregnant women in Ga-rankuwa, South Africa. BMC Res Notes 2015;8:364.

- Poyart C, Jardy L, Quesne G, Berche P, Trieu-Cuot P. Genet-ic basis of antibiotic resistance in Streptococcus agalacti-ae strains isolated in a French hospital. Antimicrob Agents Chemother 2003;47(2):794–7.

- Al-Mayahie SM. Phenotypic and genotypic comparison of ESBL production by vaginal Escherichia coli isolates from pregnant and non-pregnant women. Ann Clin Microbiol Antimicrob. 2013; 12: 7.

- Wesseling CMJ, Martin NI. Synergy by Perturbing the Gram-Negative Outer Membrane: Opening the Door for Gram-Positive Specific Antibiotics. ACS Infect Dis. 2022;8(9):1731-1757.

- Allemailem KS. A Comprehensive Computer Aided Vaccine Design Approach to Propose a Multi-Epitopes Subunit Vac-cine against Genus Klebsiella Using Pan-Genomics, Reverse Vaccinology, and Biophysical Techniques. Vaccines (Basel). 2021;9(10):1087.

- Firoozeh F, Mahluji Z, Khorshidi A, Zibaei M. Molecular char-acterization of class 1, 2 and 3 integrons in clinical multi-drug resistant Klebsiella pneumoniae isolates. Antimicrob Resist Infect Control 2019;8(59):1-7

- Czajkowski K, Broś-Konopielko M, Teliga-Czajkowska J. Urinary tract infection in women. Prz Menopauzalny. 2021;20(1):40-47.

Downloads

Published

2023-03-30

How to Cite

(1)
Dinç, K.; Akyüz, S. Agents Isolated from Vaginal Cultures in the Reproductive Period and Their Antibiotic Sensitivities (Vaginal Culture and Antibiotic Sensitivitie). Eur J Ther 2023, 29, 55-59.

Issue

Section

Original Articles