Agents Isolated from Vaginal Cultures in the Reproductive Period and Their Antibiotic Sensitivities (Vaginal Culture and Antibiotic Sensitivitie)

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vaginal discharge, vaginitis, antibiotic sensitivity


Introduction: In our study, we aimed to examine the strains isolated from vaginal swab samples sent to our laboratory from various clinics with a pre-diagnosis of vulvovaginitis and antibiotic resistance rates,  retrospectively.

Methods: ethods: 90 vaginal swab samples of patientswere included into this study. Two samples were taken from each patient and one sample was used for Gram staining. The other sample was inoculated in 5% sheep blood, Eozine Methylene Blue agar and Saboraud dextrose agar. TIdentification of isolated colonies and antibiotic susceptibility studies were carried out with Vitek 2 Compact automated system. Result of the susceptibility testing are reported according to EUCAST criteria. Nugent scoring was used for bacterial vaginosis.

Results: Normal vaginal flora elements were found in 66 (73.3 %) of the specimens, and 28 organisms were found in 24 (26.7 %). The distribution of the organisms is as follows: 6 (21.4%) Escherichia coli, 5(17.9%) Streptococcus agalactiae,  5 (17.9%) Gardnerella vaginalis, 3 (10.7%) Candida spp., 3(10.7%) Klebsiella pneumoniae, 3(10.7%) Enterococcus faecalis. Two of the E.coli strains and one of the K.pneumoniae strains are ESBL positive. Of the agents, 21 were isolated from outpatients and 7 from inpatients.  All S. agalactiae strains were susceptible to penicillin and ampicillin. E.coli and K.pneumoniae strains, which are the most frequently isolated Gram (-) organisms, are most susceptible to amikacin, carbapenems and tigecycline.

Conclusion: In patients with vaginal discharge and itching complaints, determining the organisms with microbiological culture and oriented treatment instead of empirical treatment will be more beneficial for cure.


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- FNeal CM, Kus LH, Eckert LO, Peipert JF. Noncandidal vagi-nitis: a comprehensive approach to diagnosis and manage-ment. Am J Obstet Gynecol. 2020;222(2):114-122.

- Hillier SL, Austin M, Macio I, Meyn LA, Badway D, Beigi

R. Diagnosis and Treatment of Vaginal Discharge Syn-dromes in Community Practice Settings. Clin Infect Dis. 2021;72(9):1538-1543.

- Oliveira AS, Rolo J, Gaspar C, Palmeira de Oliveira R, Marti-nez de Oliveira J, Palmeira de Oliveira A. Allergic vulvovag-initis: a systematic literature review. Arch Gynecol Obstet. 2022;306(3):593-622

- Mills BB. Vaginitis: Beyond the Basics. Obstet Gynecol Clin North Am. 2017;44(2):159-177.

- American College of Obstetricians and Gynecologists’ Com-mittee on Practice Bulletins—Gynecology. Diagnosis and Management of Vulvar Skin Disorders: ACOG Practice Bulletin, Number 224. Obstet Gynecol. 2020;136(1):e1-e14.

- Nenadić D, Pavlović MD. Value of bacterial culture of vaginal swabs in diagnosis of vaginal infections. Vojnosanit Pregl. 2015;72(6):523-528.

- EUCAST. European Committee on Antimicrobial Susceptibil-ity Testing Breakpoint tables for interpretation of MICs and zone diameters European Committee on Antimicrobial Sus-ceptibility Testing Breakpoint ts for interpretation of MICs and zone diameters. Eur Comm Antimicrob Susceptibility Testing Break tables Interpret MICs Zo diameters Version 80, 2018 http//;0-77.

- Powell AM, Nyirjesy P. Recurrent vulvovaginitis. Best Pract Res Clin Obstet Gynaecol 2014;28(7):967–976.

- Diop K, Dufour JC, Levasseur A, Fenollar F. Exhaustive rep-ertoire of human vaginal microbiota. Human Microbiome Journal 2019;11:1-7.

- Green KA, Zarek SM, Catherino WH. Gynecologic health and disease in relation to the microbiome of the female repro-ductive tract. Fertil Steril. 2015;104(6):1351–1357.

- Lopes dos Santos Santiago G, Tency I, et al. Longitudinal qPCR study of the dynamics of L. crispatus, L. iners, A. vagi-nae, (Sialidase Positive) G. vaginalis, and P. bivia in the vagi-na. PLoS One 2012; 7(9)

- Baek JC, Jo HC, Lee SM, Park JE, Cho IA, Sung JH. Prevalence of Pathogens and Other Microorganisms in Premenopaus-al and Postmenopausal Women with Vulvovaginal Symp-toms: A Retrospective Study in a Single Institute in South Korea. Medicina (Kaunas). 2021;57(6):577.

- Machado A, Almeida C, Salgueiro D, et al. Fluorescence in situ hybridization method using Peptide Nucleic Acid probes for rapid detection of Lactobacillus and Gardnerella spp. BMC Microbiol 2013;13(1).

- Kadir MA, Sulyman MA, Dawood IS, Shams-Eldin S. Tricho-monas vaginalis and associated microorganisms in wom-en with vaginal discharge in Kerkuk-Iraq. Ankara Med J 2014;14(3):91–9.

- Bolukaoto JY, Monyama CM, Chukwu MO, Lekala SM, Nch-abeleng M, Maloba MRB, vd. Antibiotic resistance of Strep-tococcus agalactiae isolated from pregnant women in Ga-rankuwa, South Africa. BMC Res Notes 2015;8:364.

- Poyart C, Jardy L, Quesne G, Berche P, Trieu-Cuot P. Genet-ic basis of antibiotic resistance in Streptococcus agalacti-ae strains isolated in a French hospital. Antimicrob Agents Chemother 2003;47(2):794–7.

- Al-Mayahie SM. Phenotypic and genotypic comparison of ESBL production by vaginal Escherichia coli isolates from pregnant and non-pregnant women. Ann Clin Microbiol Antimicrob. 2013; 12: 7.

- Wesseling CMJ, Martin NI. Synergy by Perturbing the Gram-Negative Outer Membrane: Opening the Door for Gram-Positive Specific Antibiotics. ACS Infect Dis. 2022;8(9):1731-1757.

- Allemailem KS. A Comprehensive Computer Aided Vaccine Design Approach to Propose a Multi-Epitopes Subunit Vac-cine against Genus Klebsiella Using Pan-Genomics, Reverse Vaccinology, and Biophysical Techniques. Vaccines (Basel). 2021;9(10):1087.

- Firoozeh F, Mahluji Z, Khorshidi A, Zibaei M. Molecular char-acterization of class 1, 2 and 3 integrons in clinical multi-drug resistant Klebsiella pneumoniae isolates. Antimicrob Resist Infect Control 2019;8(59):1-7

- Czajkowski K, Broś-Konopielko M, Teliga-Czajkowska J. Urinary tract infection in women. Prz Menopauzalny. 2021;20(1):40-47.




How to Cite

Dinç, K.; Akyüz, S. Agents Isolated from Vaginal Cultures in the Reproductive Period and Their Antibiotic Sensitivities (Vaginal Culture and Antibiotic Sensitivitie). Eur J Ther 2023, 29, 55-59.



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