Importance Of Polymorphisms Of The MIF Gene At Acute Leukemia And Its Effect On Febrile Neutropenic Attacks


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Authors

  • Sebahat Güven Ege Üniversitesi Fen Fakültesi Biyokimya Bölümü
  • Mustafa Pehlivan Gaziantep Üniversitesi Tıp Fakültesi Hematoloji BD
  • Mehmet Yılmaz Gaziantep Üniversitesi Tıp Fakültesi Hematoloji BD
  • Vahap Okan Gaziantep Üniversitesi Tıp Fakültesi Hematoloji BD
  • Sacide Pehlivan Gaziantep Üniversitesi Tıp Fakültesi Tıbbi Biyoloji AD

DOI:

https://doi.org/10.58600/eurjther.2009-15-2-1297-arch

Keywords:

Macrophage Migration Inhibitory Factor Gene, MIF-173 G/C polymorphism, PCR, Acute Leukemia, DNA

Abstract

The objective of this study is to research polymorphisms in MIF gene -173 site in variable haemotological malignities and evaluate its effects on febrile neutropenic attacks after chemotherapy in acute leukemia. Single nucleotide polymorphisms (SNPs) at -173 site of the MIF gene was analysed with the PCR-RFLP method (restriction endonuclease Alu I) with isolated DNA samples from 48 patients who were treated for ALL and AML and 53 healthy controls, and G and C allel frequencies were compared statistically between patients and healthy controls groups. As a result of the analyzis, it was observed that the rate of patients with GG genotypes was 70.9%, with GC genotypes 29.1%, and with CC genotypes 0%. Association is evaluated between MIF pozitivities in acute leukemias and distribution of infections, its factors, duration of feveral days and neutropenia. Within the GC allel group; no significant differences were observed (p>0.05) in infection factors, duration of neutropenia (<500/L), duration of feveral days, fungal pnomoni, blood infections and FN mortality compared to the GG allel group. In conclusion, fungal pneumonia was observed at a higher rate in the CG allel group (22%) compared to the GG allel group (11.5%); a repeat of the study with an expended group can yield more information about this relationship.

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Published

2009-06-01

How to Cite

Güven, S., Pehlivan, M., Yılmaz, M., Okan, V., & Pehlivan, S. (2009). Importance Of Polymorphisms Of The MIF Gene At Acute Leukemia And Its Effect On Febrile Neutropenic Attacks. European Journal of Therapeutics, 15(2), 5–9. https://doi.org/10.58600/eurjther.2009-15-2-1297-arch

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Section

Clinical Research