European Journal of Therapeutics
Original Investigation

Melatonin-lycopene combination improves methotrexate-induced liver toxicity in rats via anti-inflammatory effect


Department of Clinical Biochemistry, Faculty of Medicine, Harran University, Sanliurfa, Turkey


Department of General Surgery, Faculty of Medicine, Harran University, Sanliurfa, Turkey


Department of Pathology, Faculty of Medicine, Harran University, Sanliurfa, Turkey

Eur J Ther 2016; 22: 129-135
DOI: 10.5152/EurJTher.2016.004
Read: 927 Downloads: 365 Published: 03 September 2019


Introduction: This study aimed to investigate the effect of melatonin (MLT)-lycopene (LYC) combination on methotrexate-induced hepatotoxicity (MIT).


Materials and Methods: Twenty-seven Sprague-Dawley adult male rats were divided into 3 equal groups: Group I= control group, fed with oral corn oil; Group II, a single dose of methotrexate (MTX) was injected into the rats and they were fed with oral corn oil; Group III, rats were treated with a melatonin-lycopene combination for 5 days after a single dose of MTX intraperitoneal. Serum aspartate and alanine aminotransferase activities were measured as hepatic function tests. The supernatant fraction of the liver tissue homogenate was used to measure the levels of total antioxidant capacity (TAC) and total oxidant status (TOS) in order to assess the presence of oxidative stress, tumour necrosis factoralpha, and interleukin 1beta, which in turn indicate the presence of inflammation. Five sections from each rat liver tissue were prepared for histopathological examination and stained with hemotoxileosin. Then the specimens were examined under a light microscope and was evaluated semi-quantitatively.


Results: This study showed that serum aspartate aminotransferase and tissue proinflammatory cytokines such as tumour necrosis factoralpha and interleukin 1beta increased with MTX use and decreased significantly when using a combination of MLT-LYC, but oxidative stress parameters such as TOS and OSI did not change in MIT.


Conclusion: The LYC-MLT combination improved the acute toxic effects of MTX on the liver. It improved both liver function and the histopathology level. However, these effects seem to be associated with the anti-inflammatory effect than the reduction of oxidative stress.

EISSN 2564-7040