European Journal of Therapeutics
Original Article

Ischemia-Reperfusion Injury of Sciatic Nerve in Rats and Protective Role of Benidipine Hydrochloride

1.

Department of Neurosurgery, Erzincan Binali Yıldırım University, Faculty of Medicine, Erzincan, Turkey

2.

Department of Neurosurgery, Adıyaman University Medical Faculty, Adıyaman, Turkey

3.

Department of Histology and Embryology, Erzincan Binali Yıldırım University Medical Faculty, Erzincan, Turkey

4.

Department of Anatomy, Erzincan Binali Yıldırım University, Faculty of Medicine, Erzincan, Turkey

5.

Department of Biostatistics, Erzincan Binali Yıldırım University, Faculty of Medicine, Erzincan, Turkey

6.

Department of Pharmacology, Erzincan Binali Yıldırım University, Faculty of Medicine, Erzincan, Turkey

Eur J Ther 2021; 27: 286-292
DOI: 10.5152/eurjther.2021.21031
Read: 141 Downloads: 29 Published: 28 December 2021

Objective: Benidipine is an antihypertansive agent which elicits vasodilatation of arteries by blocking Ca2þ. The aim of this study is to investigate the neuroprotective effects of benidipine on rat sciatic nerves exposed to ischemia and reperfusion (I/R).

Method: 30 male Wistar Albino rats were used in this study. The rats were divided into three groups(n=10 per group). I/R procedure was administered to the IR group, 10 lg/kg/day, i.v. benidipine was given for 2 hours (h) to the BIR group before I/R procedure. And a sham group (SG) was created. Next, histopathological and biochemical investigations were performed on sciatic nerve tissues. Superoxide dismutase (SOD), Malondialdehyde (MDA) and glutathione (GSH) were analyzed as oxidative stress markers; interleukin 1 beta (IL-1b) and tumor necrosis factor alpha (TNF-a) were analyzed as inflammatory stress markers in biochemical tests.

Results: Axonal swelling, myelin loss and disorganisation were seen in the IR group. Schwann cells showed hypertrophy, hyperplasia, blood vessels were seen as congested. But these results were under normal values and almost similiar between BIR and SG groups. In the BIR group ischemic fibere degeneration score was significiantly lower than IR group. And MDA, TNF-a, IL-1b values higher, GSH and SOD values were lower in the IR group, but in the BIR group all these values were kept within normal limits.

Conclusion: This study showed that benidipine reduced oxidative stress and inflammation in rat sciatic nerve after I/R injury. 

How to cite: Çakir T, Yu¨cetas SC, Yazici GN, et al. Ischemia-Reperfusion Injury of Sciatic Nerve in Rats and Protective Role of Benidipine Hydrochloride. Eur J Ther 2021; 27(4): 286-292.

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