European Journal of Therapeutics
Original Article

Is It Possible to Regain Lost Minerals to Initial Enamel Lesions Using Enamel Matrix Derivatives Combined with Casein Phosphopeptide Amorphous Calcium Phosphate: An In Vitro Study

1.

Department of Pediatric Dentistry, Gaziantep University Faculty of Dentistry, Gaziantep, Turkey

2.

Department of Pediatric Dentistry, Ordu University Faculty of Dentistry, Ordu, Turkey

3.

Department of Pediatric Dentistry, Selçuk University Faculty of Dentistry, Konya, Turkey

Eur J Ther 2020; 26: 172-177
DOI: 10.5152/EurJTher.2020.19110
Read: 78 Downloads: 77 Published: 13 July 2020

Objevtive: This study aimed to assess the efficacy of enamel matrix derivatives (EMD) used in combination with casein phosphopeptide amorphous calcium phosphate (CPP-ACP) to recover minerals to enamel after artificial caries formation.
 

Methods: Fifty enamel samples were prepared from buccal surfaces of extracted human third molars. The samples were divided into five groups: (G1) Untreated enamel samples (control), (G2) Application of 500 ppm sodium fluoride (NaF), (G3) CPP-ACP, (G4) EMD and (G5) CPP-ACP + EMD. All of the samples were placed in a acidic buffer solution for 96 hours to simulate a carious lesion. Ca/P ratios were calculated by energy dispersive X-ray spectroscopy (EDX). After seven days of remineralization procedure, mineral contents of the samples were re-measured. All data was analyzed statistically.
 

Results: There were no statistically significant differences in the mineral contents of samples between the groups after demineralization (p>0.05). After remineralization, the Ca/P ratios of G2, G3, G4 and G5 increased significantly (p<0.05). The highest levels of Ca/P ratio were measured in G5.
 

Conclusion: Within limitation of this in vitro study, combined use of CPP-ACP and EMD may increase the remineralization potential. Furthermore, this procedures may be an alternative for providing the remineralization of enamel in future clinical trials.

How to cite: Keskin G, Güler Ç, Yıldırım S. Is it Possible to Regain Lost Minerals to Initial Enamel Lesions Using Enamel Matrix Derivatives Combined with Casein Phosphopeptide Amorphous Calcium Phosphate: An in Vitro Study. Eur J Ther 2020; 26(3): 172-7.

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