European Journal of Therapeutics
Original Article

Investigation of Bacterial Presence in Cerebrospinal Fluid by Bioimpedance Technique

1.

Department of Biophysics, SANKO University School of Medicine, Gaziantep, Turkey

2.

Department of Medical Microbiology, SANKO University School of Medicine, Gaziantep, Turkey

3.

Department of Biostatistics, SANKO University School of Medicine, Gaziantep, Turkey

Eur J Ther 2020; 26: 208-213
DOI: 10.5152/eurjther.2020.20136
Read: 57 Downloads: 63 Published: 22 September 2020

Objective: This study aimed to determine the presence of bacteria and their colony numbers in cerebrospinal fluid (CSF) by bioimpedance measurements at 50 kHz phase angle (PA) and 5 kHz impedance values.

Methods: We evaluated the performance of the 18 Gauge probe for detecting several types of bacteria in CSF ex-vivo. We equally monitored the electrical differentiation between colony numbers. A quantity of 200 µl sterile CSF was used as the standard in each experiment and was inoculated with 1, 2, 5, and 10 colonies of Coagulase-negative staphylococci, Streptococcus pneumoniae and Acinetobacter baumannii, separately.

Results: PA and impedance values of CSF samples were compared with each other concerning different colony numbers. It was observed that, varying numbers of colony strains of Acinetobacter baumannii, Coagulase-negative staphylococci, and Streptococcus pneumoniae could be differentiated from sterile CSF using PA and impedance values. Only one colony of the A. baumannii strain could not be distinguished from sterile CSF due to its thin cell wall composition.

Conclusion: The bioimpedance probe was time-saving and could detect the presence of bacteria in CSF samples correctly. Moreover, the probe can be used in the rapid detection of bacteria in CSF during real-time examinations.

How to cite: Denkçeken T, Atak C, Demirbakan H, Erdoğan M, Günel Karadeniz P. Investigation of bacterial presence in cerebrospinal fluid by bioimpedance technique. Eur J Ther 2020; 26(3): 208-13.

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EISSN 2564-7040