ISSN 2564-7784 | E-ISSN 2564-7040
Original Article
The Protective Effect of Proanthocyanidin Against Methotrexate-Induced Testicle Damage in Rats
1 Department of Histology and Embryology, Gaziantep University School of Medicine, Gaziantep, Turkey  
2 Department of Histology and Embryology, Çukurova University School of Medicine, Adana, Turkey  
Eur J Ther ; : -
DOI: 10.5152/EurJTher.2018.790
Key Words: Methotrexate, proanthocyanidin, testis
Abstract

Objective: Methotrexate is widely used chemotherapeutic agent that has side effects such as hepatotoxicity and also testicular damage by increasing the production of reactive oxygen species. Proanthocyanidins are known as natural antioxidants that have protective effects against oxidative stress. This study intends to investigate the protective effect of proanthocyanidin against methotrexate-induced testicular damage.


Methods
: 26 male Wistar Albino rats were divided into 4 groups. Saline (2cc/kg) was administered to control group and Proanthocyanidin (200mg/kg) were administered to proanthocyanidin group for 7 days. Methotrexate group received single dose of 20mg/kg methotrexate on day 3. 200mg/kg of proanthocyanidin for 6 days was administered to proanthocyanidin+methotrexate group and the group also received single dose of 20mg/kg methotrexate on day 3. Animals were sacrificed on day 8. SOD, GPX, MDA analyses were performed, besides light and electron microscopic examinations on the testis samples.


Results
: Histopathological findings such as thinning of the seminiferous tubule epithelium and decrease in spermatogonial cells were detected in methotrexate group. Electron microscopic findings indicated similar damage. MDA levels increased in testicular tissues, while SOD and GPX levels decreased significantly. Tissue damage and deterioration in biochemical parameters were decreased in proanthocyanidin+methotrexate group.


Conclusion
: The results show that proanthocyanidin does not completely prevent damage to the testicles caused by methotrexate but has important protective effect.

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